Multi-omics quantitative data of tomato fruit unveils regulation modes of least variable metabolites.

Autor: Moing A; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France. annick.moing@inrae.fr.; Bordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France. annick.moing@inrae.fr., Berton T; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France., Roch L; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France., Diarrassouba S; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; Laboratoire de Recherche en Sciences Végétales, UMR 5546 UPS/CNRS, Auzeville- Tolosane, F-31320, France., Bernillon S; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; Bordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; INRAE, Mycologie et Sécurité des Aliments, UR 1264, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France., Arrivault S; Max Planck Institute of Molecular Plant Physiology, am Muehlenberg 14476, Potsdam-Golm, Germany., Deborde C; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; Bordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; INRAE, UR1268 BIA, Centre INRAE Pays de Loire - Nantes, Nantes, F-44000, France.; Present address: INRAE, BIBS Facility, Centre INRAE Pays de Loire - Nantes, Nantes, F-44000, France., Maucourt M; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; Bordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France., Cabasson C; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; Bordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France., Bénard C; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France., Prigent S; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; Bordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France., Jacob D; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; Bordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France., Gibon Y; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France.; Bordeaux Metabolome, MetaboHUB, PHENOME-EMPHASIS, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France., Lemaire-Chamley M; INRAE, Univ. Bordeaux, Biologie du Fruit et Pathologie, UMR 1332, Centre INRAE de Nouvelle Aquitaine Bordeaux, Villenave d'Ornon, F-33140, France. martine.lemaire@inrae.fr.
Jazyk: angličtina
Zdroj: BMC plant biology [BMC Plant Biol] 2023 Jul 22; Vol. 23 (1), pp. 365. Date of Electronic Publication: 2023 Jul 22.
DOI: 10.1186/s12870-023-04370-0
Abstrakt: Background: The composition of ripe fruits depends on various metabolites which content evolves greatly throughout fruit development and may be influenced by the environment. The corresponding metabolism regulations have been widely described in tomato during fruit growth and ripening. However, the regulation of other metabolites that do not show large changes in content have scarcely been studied.
Results: We analysed the metabolites of tomato fruits collected on different trusses during fruit development, using complementary analytical strategies. We identified the 22 least variable metabolites, based on their coefficients of variation. We first verified that they had a limited functional link with the least variable proteins and transcripts. We then posited that metabolite contents could be stabilized through complex regulations and combined their data with the quantitative proteome or transcriptome data, using sparse partial-least-square analyses. This showed shared regulations between several metabolites, which interestingly remained linked to early fruit development. We also examined regulations in specific metabolites using correlations with individual proteins and transcripts, which revealed that a stable metabolite does not always correlate with proteins and transcripts of its known related pathways.
Conclusions: The regulation of the least variable metabolites was then interpreted regarding their roles as hubs in metabolic pathways or as signalling molecules.
(© 2023. The Author(s).)
Databáze: MEDLINE
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