Impact of the Probiotic Organism Megasphaera elsdenii on Escherichia coli O157:H7 Prevalence in Finishing Cattle.

Autor:	Maher JM; Department of Animal Sciences and Industry, Kansas State University, 1530 Mid-Campus Drive North, Manhattan, KS 66506, USA., Drouillard JS; Department of Animal Sciences and Industry, Kansas State University, 1530 Mid-Campus Drive North, Manhattan, KS 66506, USA., Baker AN; Department of Animal Sciences and Industry, Kansas State University, 1530 Mid-Campus Drive North, Manhattan, KS 66506, USA., de Aguiar Veloso V; Department of Animal Sciences and Industry, Kansas State University, 1530 Mid-Campus Drive North, Manhattan, KS 66506, USA., Kang Q; Department of Statistics, Kansas State University, 1116 Mid-Campus Drive North, Manhattan, KS 66506, USA., Kastner JJ; Department of Diagnostic Medicine and Pathobiology, Kansas State University, 1620 Denison Avenue, Manhattan, KS 66506, USA., Gragg SE; Department of Animal Sciences and Industry, Kansas State University, 1530 Mid-Campus Drive North, Manhattan, KS 66506, USA. Electronic address: saragragg@k-state.edu.
Jazyk:	angličtina
Zdroj:	Journal of food protection [J Food Prot] 2023 Sep; Vol. 86 (9), pp. 100133. Date of Electronic Publication: 2023 Jul 20.
DOI:	10.1016/j.jfp.2023.100133
Abstrakt:	Feedlot cattle commonly shed the foodborne pathogen Escherichia coli O157:H7 in their feces. Megasphaera elsdenii (ME), a lactic acid-utilizing bacterium, is commonly administered to cattle to avoid lactate accumulation in the rumen and to control ruminal acidosis. The impact of administering ME on foodborne pathogen prevalence, specifically E. coli O157:H7, has not been explored. The purpose of this study was to quantify E. coli O157:H7 prevalence in finishing cattle administered ME. Cattle (n = 448) were assigned to treatments in a randomized complete block design with repeated measurements over two sampling periods. Treatments were arranged as a 2 × 2 factorial containing: ruminally protected lysine (RPL; included for a complementary study) fed at 0% or 0.45% of diet dry matter; with or without ME. Freeze-dried ME was administered as an oral drench (1 × 10 10 CFU/steer on day one) and then top dressed onto basal diets (1 × 10 7 CFU/steer) daily thereafter. Rectoanal mucosal swabs (RAMS) were obtained from animals before harvest to determine the E. coli O157:H7 prevalence. The inclusion of RPL (P = 0.2136) and ME (P = 0.5012) did not impact E. coli O157:H7 prevalence, and RPL was not included in any significant interactions (P > 0.05). A significant interaction was observed between ME and sampling period (P = 0.0323), indicating that the effect of ME on E. coli O157:H7 prevalence varied over the sampling period. A diet containing ME reduced the odds of E. coli O157:H7 prevalence by 50% during sampling period 1 (8.0% and 14.7% for cattle with and without ME, respectively) and increased the odds by 23% during sampling period 2 (10.8% and 8.9% for cattle with and without ME, respectively). Administering ME in cattle diets did not impact E. coli O157:H7 in feedlot cattle. This is the first study to investigate the use of ME as a preharvest food safety intervention in cattle, and additional research is necessary to determine the efficacy. Competing Interests: Declaration of Competing Interest Sara Gragg reports financial support and equipment, drugs, or supplies were provided by MS Biotec, LLC. James Drouillard reports a relationship with MS Biotec, LLC. that includes: equity or stocks. James Drouillard has patent issued to MS Biotec, LLC. Editorial Board Member – Sara Gragg. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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