Genetic and Functional Analyses of Cutibacterium Acnes Isolates Reveal the Association of a Linear Plasmid with Skin Inflammation.

Autor:	O'Neill AM; Department of Dermatology, University of California San Diego, La Jolla, California, USA., Cavagnero KJ; Department of Dermatology, University of California San Diego, La Jolla, California, USA., Seidman JS; Department of Cellular & Molecular Medicine, University of California San Diego, La Jolla, California, USA., Zaramela L; Department of Pediatrics, School of Medicine, University of California San Diego, La Jolla, California, USA., Chen Y; Department of Dermatology, University of California San Diego, La Jolla, California, USA; Department of Pediatrics, School of Medicine, University of California San Diego, La Jolla, California, USA., Li F; Department of Dermatology, University of California San Diego, La Jolla, California, USA., Nakatsuji T; Department of Dermatology, University of California San Diego, La Jolla, California, USA., Cheng JY; Department of Dermatology, University of California San Diego, La Jolla, California, USA., Tong YL; Department of Dermatology, University of California San Diego, La Jolla, California, USA., Do TH; Division of Dermatology, School of Medicine, University of California Los Angeles, Los Angeles, California, USA., Brinton SL; Department of Dermatology, University of California San Diego, La Jolla, California, USA., Hata TR; Department of Dermatology, University of California San Diego, La Jolla, California, USA., Modlin RL; Division of Dermatology, School of Medicine, University of California Los Angeles, Los Angeles, California, USA., Gallo RL; Department of Dermatology, University of California San Diego, La Jolla, California, USA. Electronic address: rgallo@health.ucsd.edu.
Jazyk:	angličtina
Zdroj:	The Journal of investigative dermatology [J Invest Dermatol] 2024 Jan; Vol. 144 (1), pp. 116-124.e4. Date of Electronic Publication: 2023 Jul 20.
DOI:	10.1016/j.jid.2023.05.029
Abstrakt:	Cutibacterium acnes is a commensal bacterium on the skin that is generally well-tolerated, but different strain types have been hypothesized to contribute to the disease acne vulgaris. To understand how some strain types might contribute to skin inflammation, we generated a repository of C. acnes isolates from skin swabs of healthy subjects and subjects with acne and assessed their strain-level identity and capacity to stimulate cytokine release. Phylotype II K-type strains were more frequent on healthy and nonlesional skin of subjects with acne than those isolated from lesions. Phylotype IA-1 C-type strains were increased on lesional skin compared with those on healthy skin. The capacity to induce cytokines from cultured monocyte-derived dendritic cells was opposite to this action on sebocytes and keratinocytes and did not correlate with the strain types associated with the disease. Whole-genome sequencing revealed a linear plasmid in high-inflammatory isolates within similar strain types that had different proinflammatory responses. Single-cell RNA sequencing of mouse skin after intradermal injection showed that strains containing this plasmid induced a higher inflammatory response in dermal fibroblasts. These findings revealed that C. acnes strain type is insufficient to predict inflammation and that carriage of a plasmid could contribute to disease. (Copyright © 2023. Published by Elsevier Inc.)
Databáze:	MEDLINE
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