Rose bengal-encapsulated chitosan nanoparticles for the photodynamic treatment of Trichophyton species.

Autor: Bekmukhametova A; School of Science, Western Sydney University, Penrith, New South Wales, Australia., Antony A; School of Medicine, Western Sydney University, Penrith, New South Wales, Australia., Halliday C; Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, Westmead Hospital, Westmead, New South Wales, Australia., Chen S; Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, Westmead Hospital, Westmead, New South Wales, Australia.; Sydney Medical School, University of Sydney, Westmead, New South Wales, Australia., Ho CH; School of Science, Western Sydney University, Penrith, New South Wales, Australia.; School of Biomedical Sciences, The University of Queensland, Brisbane, Queensland, Australia., Uddin MMN; School of Science, Western Sydney University, Penrith, New South Wales, Australia.; Department of Pharmacy, Faculty of Biological Science, University of Chittagong, Chittagong, Bangladesh., Longo L; School of Medicine, University of Siena, Siena, Italy., Pedrinazzi C; School of Medicine, University of Pavia, Pavia, Italy., George L; Advanced Materials Characterisation Facility (AMCF), Western Sydney University, Penrith, New South Wales, Australia., Wuhrer R; Advanced Materials Characterisation Facility (AMCF), Western Sydney University, Penrith, New South Wales, Australia., Myers S; School of Medicine, Western Sydney University, Penrith, New South Wales, Australia., Mawad D; School of Materials Science and Engineering, University of New South Wales, Kensington, New South Wales, Australia.; Australian Centre for NanoMedicine, UNSW Australia, Sydney, New South Wales, Australia., Houang J; School of Science, Western Sydney University, Penrith, New South Wales, Australia., Lauto A; School of Science, Western Sydney University, Penrith, New South Wales, Australia.; Biomedical Engineering & Neuroscience Research Group, The MARCS Institute, Western Sydney University, Penrith, New South Wales, Australia.
Jazyk: angličtina
Zdroj: Photochemistry and photobiology [Photochem Photobiol] 2024 Jan-Feb; Vol. 100 (1), pp. 115-128. Date of Electronic Publication: 2023 Jul 21.
DOI: 10.1111/php.13839
Abstrakt: Rose bengal (RB) solutions coupled with a green laser have proven to be efficient in clearing resilient nail infections caused by Trichophyton rubrum in a human pilot study and in extensive in vitro experiments. Nonetheless, the RB solution can become diluted or dispersed over the tissue and prevented from penetrating the nail plate to reach the subungual area where fungal infection proliferates. Nanoparticles carrying RB can mitigate the problem of dilution and are reported to effectively penetrate through the nail. For this reason, we have synthesized RB-encapsulated chitosan nanoparticles with a peak distribution size of ~200 nm and high reactive oxygen species (ROS) production. The RB-encapsulated chitosan nanoparticles aPDT were shown to kill more than 99% of T. rubrum, T. mentagrophytes, and T. interdigitale spores, which are the common clinically relevant pathogens in onychomycosis. These nanoparticles are not cytotoxic against human fibroblasts, which promotes their safe application in clinical translation.
(© 2023 The Authors. Photochemistry and Photobiology published by Wiley Periodicals LLC on behalf of American Society for Photobiology.)
Databáze: MEDLINE
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