Real-time cell metabolism assessed repeatedly on the same cells via para-hydrogen induced polarization.

Autor:	Ding Y; Group of NMR Signal Enhancement Max Planck Institute for Multidisciplinary Sciences Am Fassberg 11 37077 Göttingen Germany stefan.gloeggler@mpinat.mpg.de.; Center for Biostructural Imaging of Neurodegeneration University Medical Center Göttingen Von-Siebold-Str. 3A 37075 Göttingen Germany., Stevanato G; Group of NMR Signal Enhancement Max Planck Institute for Multidisciplinary Sciences Am Fassberg 11 37077 Göttingen Germany stefan.gloeggler@mpinat.mpg.de.; Center for Biostructural Imaging of Neurodegeneration University Medical Center Göttingen Von-Siebold-Str. 3A 37075 Göttingen Germany., von Bonin F; Clinic for Hematology and Medical Oncology University Medical Center Göttingen Robert-Koch-Str. 40 37075 Göttingen Germany., Kube D; Clinic for Hematology and Medical Oncology University Medical Center Göttingen Robert-Koch-Str. 40 37075 Göttingen Germany., Glöggler S; Group of NMR Signal Enhancement Max Planck Institute for Multidisciplinary Sciences Am Fassberg 11 37077 Göttingen Germany stefan.gloeggler@mpinat.mpg.de.; Center for Biostructural Imaging of Neurodegeneration University Medical Center Göttingen Von-Siebold-Str. 3A 37075 Göttingen Germany.
Jazyk:	angličtina
Zdroj:	Chemical science [Chem Sci] 2023 Jun 20; Vol. 14 (28), pp. 7642-7647. Date of Electronic Publication: 2023 Jun 20 (Print Publication: 2023).
DOI:	10.1039/d3sc01350b
Abstrakt:	Signal-enhanced or hyperpolarized nuclear magnetic resonance (NMR) spectroscopy stands out as a unique tool to monitor real-time enzymatic reactions in living cells. The singlet state of para-hydrogen is thereby one source of spin order that can be converted into largely enhanced signals of e.g. metabolites. Here, we have investigated a parahydrogen-induced polarization (PHIP) approach as a biological assay for in vitro cellular metabolic characterization. Here, we demonstrate the possibility to perform consecutive measurements yielding metabolic information on the same sample. We observed a strongly reduced pyruvate-to-lactate conversion rate (flux) of a Hodgkin's lymphoma cancer cell line L1236 treated with FK866, an inhibitor of nicotinamide phosphoribosyltransferase (NAMPT) affecting the amount of NAD + and thus NADH in cells. In the consecutive measurement the flux was recovered by NADH to the same amount as in the single-measurement-per-sample and provides a promising new analytical tool for continuous real-time studies combinable with bioreactors and lab-on-a-chip devices in the future. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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