The link between relative stability constant of DNA- and BSA-chromenopyrimidine complexes and cytotoxicity towards human breast cancer cells (MCF-7).

Autor: Zamisa SJ; School of Chemistry and Physics, University of KwaZulu-Natal Private Bag X54001 Durban 4000 South Africa owaga@ukzn.ac.za., Adeleke AA; School of Chemistry and Physics, University of KwaZulu-Natal Private Bag X54001 Durban 4000 South Africa owaga@ukzn.ac.za., Devnarain N; Molecular and Clinical Pharmacology Research Laboratory, Department of Pharmacology, Discipline of Pharmaceutical Science, University of KwaZulu-Natal Private Bag X54001 Durban 4000 South Africa., Rhman MA; Molecular and Clinical Pharmacology Research Laboratory, Department of Pharmacology, Discipline of Pharmaceutical Science, University of KwaZulu-Natal Private Bag X54001 Durban 4000 South Africa., Owira PMO; Molecular and Clinical Pharmacology Research Laboratory, Department of Pharmacology, Discipline of Pharmaceutical Science, University of KwaZulu-Natal Private Bag X54001 Durban 4000 South Africa., Omondi B; School of Chemistry and Physics, University of KwaZulu-Natal Private Bag X54001 Durban 4000 South Africa owaga@ukzn.ac.za.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2023 Jul 19; Vol. 13 (32), pp. 21820-21837. Date of Electronic Publication: 2023 Jul 19 (Print Publication: 2023).
DOI: 10.1039/d3ra01741a
Abstrakt: In this study, we synthesized and characterized ten chromenopyrimidine derivatives using analytical and spectroscopic methods. Studies on DNA and albumin binding affinity, as well as cytotoxicity tests on human breast cancer (MCF-7) cells, of the chromenopyrimidines, were conducted. The natural logarithm of the relative stability constant of DNA- and BSA-chromenopyrimidine complexes [ln( K DNA / K BSA )] was used as a criterion for selecting compounds for cytotoxicity studies. We found that ln( K DNA / K BSA ) was inversely related to IC 50 values of the compounds in MCF-7 cells. The antiproliferative effects of the compounds were found to induce apoptosis in MCF-7 cells, which is a desired mechanism of cell death. Correlations between the DNA and albumin binding affinities of chromenopyrimidines were established. We propose that this relationship approach can, for a given set of compounds, assist in predicting the cytotoxicity of potential drug candidates towards MCF-7 cells based on their experimentally determined CT-DNA and BSA binding affinities.
Competing Interests: The authors declare no competing interests.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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