| Autor: |
Cáceres-Jiménez S; Department of Agroindustry and Food Quality, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Alameda del Obispo, Avda. Menéndez-Pidal, Córdoba 14004, Spain.; Departamento de Bromatología y Tecnología de los Alimentos, Campus Rabanales, Ed. Darwin-anexo, Universidad de Córdoba, Córdoba 14071, Spain., Rodríguez-Solana R; Department of Agroindustry and Food Quality, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Alameda del Obispo, Avda. Menéndez-Pidal, Córdoba 14004, Spain., Dobani S; Nutrition Innovation Centre for Food and Health (NICHE), Ulster University, Coleraine BT1 6DN, U.K., Pourshahidi K; Nutrition Innovation Centre for Food and Health (NICHE), Ulster University, Coleraine BT1 6DN, U.K., Gill C; Nutrition Innovation Centre for Food and Health (NICHE), Ulster University, Coleraine BT1 6DN, U.K., Moreno-Rojas JM; Department of Agroindustry and Food Quality, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Alameda del Obispo, Avda. Menéndez-Pidal, Córdoba 14004, Spain.; Foods for Health Group, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba 14004, Spain., Almutairi TM; Department of Chemistry, King Saud University, Riyadh 11451, Saudi Arabia., Crozier A; Department of Chemistry, King Saud University, Riyadh 11451, Saudi Arabia.; School of Medicine, Dentistry and Nursing, University of Glasgow, Glasgow G12 8QQ, U.K., Pereira-Caro G; Department of Agroindustry and Food Quality, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Alameda del Obispo, Avda. Menéndez-Pidal, Córdoba 14004, Spain.; Foods for Health Group, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Córdoba 14004, Spain. |
| Abstrakt: |
After an acute intake of 300 g of mango purée by 10 subjects, 0 and 24 h urine and plasma samples were analyzed by high-performance liquid chromatography-high-resolution mass spectrometry. The method was first validated for 44 reference polyphenols in terms of linearity, specificity, limits of detection and quantification, intra-day and inter-day precision, recovery, and matrix effects in two biological matrices. After method validation, a total of 94 microbial-derived phenolic catabolites, including 15 cinnamic acids, 3 phenylhydracrylic acids, 14 phenylpropanoic acids, 12 phenylacetic acids, 28 benzoic acids, 2 mandelic acids, 15 hydroxybenzenes, and 5 hippuric acid derivatives, were identified or tentatively identified in urine and/or plasma. These results establish the value of the UHPLC-HRMS protocol and the use of authentic standards to obtain a detailed and accurate picture of mango polyphenol metabolites, together with their phase II conjugated metabolites, in human bioavailability studies. |
