Biolistic transformation of the yeast Saccharomyces cerevisiae mitochondrial DNA.

Autor: Veloso Ribeiro Franco L; Department of Microbiology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Sao Paulo, Brazil., Barros MH; Department of Microbiology, Institute of Biomedical Sciences, Universidade de Sao Paulo, Sao Paulo, Brazil.
Jazyk: angličtina
Zdroj: IUBMB life [IUBMB Life] 2023 Dec; Vol. 75 (12), pp. 972-982. Date of Electronic Publication: 2023 Jul 20.
DOI: 10.1002/iub.2769
Abstrakt: The insertion of genes into mitochondria by biolistic transformation is currently only possible in the yeast Saccharomyces cerevisiae and the algae Chlamydomonas reinhardtii. The fact that S. cerevisiae mitochondria can exist with partial (ρ - mutants) or complete deletions (ρ 0 mutants) of mitochondrial DNA (mtDNA), without requiring a specific origin of replication, enables the propagation of exogenous sequences. Additionally, mtDNA in this organism undergoes efficient homologous recombination, making it well-suited for genetic manipulation. In this review, we present a summarized historical overview of the development of biolistic transformation and discuss iconic applications of the technique. We also provide a detailed example on how to obtain transformants with recombined foreign DNA in their mitochondrial genome.
(© 2023 International Union of Biochemistry and Molecular Biology.)
Databáze: MEDLINE
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