| Autor: |
Hirose H; Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan., Nakata E; Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan., Zhang Z; Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan., Shibano Y; Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan., Maekawa M; Division of Physiological Chemistry and Metabolism, Graduate School of Pharmaceutical Sciences, Keio University, Minato, Tokyo 105-8512, Japan., Morii T; Institute of Advanced Energy, Kyoto University, Uji, Kyoto 611-0011, Japan., Futaki S; Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. |
| Abstrakt: |
A fluorescent sensor that allows simultaneous analysis of environmental factors in a limited cellular space is useful for understanding precise molecular interactions in live cells and their biological responses. Macropinocytosis is a ubiquitous endocytic pathway for massive uptake of extracellular fluids, resulting in the formation of macropinosomes. Although macropinocytosis may impact intracellular delivery and cancer proliferation, information on the intracellular behaviors of macropinosomes is limited. Here, we aimed to develop a macropinoscope, a sensor that simultaneously detects pH and cathepsin B activity in individual macropinosomes. A macropinosome-specific marker, dextran (70 kDa), was employed as a platform, onto which fluorescein, Oregon Green, and tetramethylrhodamine were loaded for ratiometric pH sensing and imaging. A cathepsin-B-cleavable peptide sequence bearing sulfo-Cy5 and the quencher BHQ-3 was also mounted; cleavage of the sequence was detected as an increase in sulfo-Cy5 fluorescence. A steep decrease in pH was observed 5-10 min after macropinosome formation, which was accompanied by an immediate increase in cathepsin B activity. Our design concept will lead to the development of other macropinoscopes for the simultaneous detection of other parameters in individual macropinosomes. |
