Ursolic acid-rich extract presents trypanocidal action in vitro but worsens mice under experimental acute Chagas disease.

Autor: Daga MA; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, Brazil., Nicolau ST; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, Brazil., Jurumenha-Barreto J; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, Brazil., Lima LBS; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, Brazil., Cabral IL; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, Brazil., Pivotto AP; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, Brazil., Stefanello A; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, Brazil., Amorim JPA; Center of Biological and Health Sciences, Western Parana State University, Cascavel, Brazil., Hoscheid J; Professional Master's Program in Medicinal Plants and Herbal Medicine in Primary Care, Universidade Paranaense, Umuarama, Brazil., Silva EA; Laboratory of Biotechnological Processes and Separation, Center of Exact and Technological Sciences, Western Parana State University, Toledo, Brazil., Ayala TS; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, Brazil., Menolli RA; Laboratory of Applied Immunology, Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel, Brazil.
Jazyk: angličtina
Zdroj: Parasite immunology [Parasite Immunol] 2023 Oct; Vol. 45 (10), pp. e13005. Date of Electronic Publication: 2023 Jul 19.
DOI: 10.1111/pim.13005
Abstrakt: Chagas disease is a neglected tropical disease with only two drugs available for treatment and the plant Cecropia pachystachya has several compounds with antimicrobial and anti-inflammatory activities. This study aimed to evaluate a supercritical extract from C. pachystachya leaves in vitro and in vivo against Trypanosoma cruzi. A supercritical CO 2 extraction was used to obtain the extract (CPE). Cytotoxicity and immunostimulation ability were evaluated in macrophages, and the in vitro trypanocidal activity was evaluated against epimastigotes and trypomastigotes forms. In vivo tests were done by infecting BALB/c mice with blood trypomastigotes forms and treating animals orally with CPE for 10 days. The parasitemia, survival rate, weight, cytokines and nitric oxide dosage were evaluated. CPE demonstrated an effect on the epi and trypomastigotes forms of the parasite (IC 50 17.90 ± 1.2 μg/mL; LC 50 26.73 ± 1.2 μg/mL) and no changes in macrophages viability, resulting in a selectivity index similar to the reference drug. CPE-treated animals had a worsening compared to non-treated, demonstrated by higher parasitemia and lower survival rate. This result was attributed to the anti-inflammatory effect of CPE, demonstrated by the higher IL-10 and IL-4 values observed in the treated mice compared to the control ones. CPE demonstrated a trypanocidal effect in vitro and a worsening in the in vivo infection due to its anti-inflammatory activity.
(© 2023 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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