| Autor: |
Lin YH; Department of Chemistry, Tunghai University, Taichung 407224, Taiwan., Singuru MMR; Department of Chemistry, Tunghai University, Taichung 407224, Taiwan., Marpaung DSS; International Ph.D. Program in Biomedical and Materials Science, Tunghai University, Taichung 407224, Taiwan.; Department of Biosystems Engineering, Institut Teknologi Sumatera, Lampung Selatan 35365, Indonesia., Liao WC; Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan., Chuang MC; Department of Chemistry, Tunghai University, Taichung 407224, Taiwan.; International Ph.D. Program in Biomedical and Materials Science, Tunghai University, Taichung 407224, Taiwan. |
| Abstrakt: |
Cargo molecule-encapsulated DNA capsules synthesized with a solid sacrificial template have elicited significant interest in the last decade and have been used for active materials in applications ranging from biosensors to drug delivery. However, the correlation between template properties and the subsequent assembly and triggered release behavior of the resultant carriers remain uninvestigated. In this study, ethylene glycol (EG) was added during the CaCO 3 precipitation synthesis to yield particles of various sizes and surface properties, and the adenosine triphosphate (ATP)-responsive release characteristics of the fabricated DNA capsules affected by these particle properties were investigated. The geometry, crystallization, and surface morphology of the CaCO 3 particles co-precipitated at various EG concentrations were characterized. We discuss the integrity of cross-linking hybridization, fluorescent molecule internalization, degree of leakage, and release efficiency of the resulting DNA capsules and their relevance brought by particle properties. To achieve efficient encapsulation and cargo release, the surface roughness of the CaCO 3 particles was explored and was deemed a key determinant of the compactness of the DNA shell after template removal. This effect was particularly strong in CaCO 3 particles in connection with high EG concentrations. The DNA capsules fabricated using 83% EG exhibited low leakage, high loading, and moderate release efficiencies as well as a greater apparent association constant with ATP due to their small particle size and the high-integrity DNA shells. |
