Concentration-dependent diffusion of unlabeled protein within an in vitro hyaluronic acid matrix.

Autor: Dos Santos ACF; Department of Agricultural and Biological Engineering, Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana, USA., Ahmadzadegan A; School of Mechanical Engineering, Purdue University, West Lafayette, Indiana, USA., Ximenes E; Department of Agricultural and Biological Engineering, Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana, USA., Vlachos P; School of Mechanical Engineering, Purdue University, West Lafayette, Indiana, USA., Ardekani A; School of Mechanical Engineering, Purdue University, West Lafayette, Indiana, USA., Kapur S; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA., Corvari V; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA., Ladisch MR; Department of Agricultural and Biological Engineering, Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana, USA.; Eli Lilly, Indianapolis, Indiana, USA.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2023 Aug; Vol. 120 (8), pp. 2326-2332. Date of Electronic Publication: 2023 Jul 19.
DOI: 10.1002/bit.28505
Abstrakt: Diffusion and movement of subcutaneously injected biologics and high-concentration immunoglobulin G (IgG) therapeutics away from the injection site and through the subcutaneous (SC) tissue may be concentration dependent. This possibility was confirmed by in situ measurement of diffusion coefficients of unlabeled bovine IgG in phosphate-buffered saline within an in vitro hyaluronic acid matrix that represents the SC electrostatic environment. Diffusion decreased from 2.67 to 0.05 × 10 -7  cm 2 /s when IgG concentration increased from 25 to 73 mg/mL. The results demonstrated that in situ detection of unlabeled proteins within an in vitro SC environment provides another useful tool for the preclinical characterization of injectable biologics.
(© 2023 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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