Mechanism and kinetic model of microalgal enzymatic hydrolysis for prospective bioethanol conversion.

Autor:	Padil; Department of Chemical Engineering, Riau University Pekanbaru 28293 Indonesia., Putra MD; Department of Chemical Engineering, Lambung Mangkurat University Banjarbaru 70713 Indonesia mdputra@ulm.ac.id., Hidayat M; Department of Chemical Engineering, Gadjah Mada University Yogyakarta 55284 Indonesia., Kasiamdari RS; Department of Biology, Gadjah Mada University Yogyakarta 55281 Indonesia., Mutamima A; Department of Chemical Engineering, Riau University Pekanbaru 28293 Indonesia., Iwamoto K; Department of Environmental Engineering and Green Technology, Universiti Technologi Malaysia Kuala Lumpur 54100 Malaysia., Darmawan MA; Research Center for Process and Manufacturing Industry Technology, Research Organization for Energy and Manufacture, National Research and Innovation Agency Jakarta Pusat 10340 Indonesia., Gozan M; Department of Chemical Engineering, University of Indonesia Depok 16424 Indonesia.; Research Center for Biomass Valorization, University of Indonesia Depok 16424 Indonesia.
Jazyk:	angličtina
Zdroj:	RSC advances [RSC Adv] 2023 Jul 17; Vol. 13 (31), pp. 21403-21413. Date of Electronic Publication: 2023 Jul 17 (Print Publication: 2023).
DOI:	10.1039/d3ra01556d
Abstrakt:	Tetraselmis chuii is a potential microalgae that is in consideration for producing bioethanol owing to its large content of carbohydrates. The glucose production from T. chuii through an enzymatic process with cellulase and xylanase (pretreatment process) and α-amylase and glucoamylase (saccharification process) was studied. The mechanism of the enzymatic process was developed and the kinetic models were then evaluated. For the pretreatment process, enzymes with 30% concentration reacted at 30 °C for 40 min resulted in 35.9% glucose yield. For the saccharification process, the highest glucose yield of 90.03% was obtained using simultaneous α-amylase (0.0006%) and glucoamylase (0.01%) enzymes at 55 °C and for 40 min. The kinetic models fitted well with the experimental data. The model also revealed that the saccharification process performed better than the pretreatment process with a higher kinetic constant and lower activation energy. The proposed kinetic model plays an important role in implementing processes at a larger scale. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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