Fiberoptic hemodynamic spectroscopy reveals abnormal cerebrovascular reactivity in a freely moving mouse model of Alzheimer's disease.

Autor: Gareau DS; Laboratory of Investigative Dermatology, The Rockefeller University, New York, NY, United States., RochaKim N; Department of Pharmacology, Physiology and Neuroscience, Rutgers-New Jersey Medical School, Newark, NJ, United States., Choudhury A; Department of Pharmacology, Physiology and Neuroscience, Rutgers-New Jersey Medical School, Newark, NJ, United States., Bamkole M; Department of Pharmacology, Physiology and Neuroscience, Rutgers-New Jersey Medical School, Newark, NJ, United States., Snuderl M; Department of Pathology, NYU Langone Health and Grossman School of Medicine, New York, NY, United States., Zou J; Laboratory of Investigative Dermatology, The Rockefeller University, New York, NY, United States., Yaroslavsky A; Department of Physics and Applied Physics, University of Massachusetts, Lowell, MA, United States., Jacques SL; Department of Bioengineering, University of Washington, Seattle, WA, United States., Strickland S; Laboratory of Neurobiology and Genetics, The Rockefeller University, New York, NY, United States., Krueger JG; Laboratory of Investigative Dermatology, The Rockefeller University, New York, NY, United States., Ahn HJ; Department of Pharmacology, Physiology and Neuroscience, Rutgers-New Jersey Medical School, Newark, NJ, United States.; Brain Health Institute, Rutgers University, Piscataway, NJ, United States.
Jazyk: angličtina
Zdroj: Frontiers in molecular neuroscience [Front Mol Neurosci] 2023 Jul 03; Vol. 16, pp. 1163447. Date of Electronic Publication: 2023 Jul 03 (Print Publication: 2023).
DOI: 10.3389/fnmol.2023.1163447
Abstrakt: Many Alzheimer's disease (AD) patients suffer from altered cerebral blood flow and damaged cerebral vasculature. Cerebrovascular dysfunction could play an important role in this disease. However, the mechanism underlying a vascular contribution in AD is still unclear. Cerebrovascular reactivity (CVR) is a critical mechanism that maintains cerebral blood flow and brain homeostasis. Most current methods to analyze CVR require anesthesia which is known to hamper the investigation of molecular mechanisms underlying CVR. We therefore combined spectroscopy, spectral analysis software, and an implantable device to measure cerebral blood volume fraction ( CBVF ) and oxygen saturation ( S O2 ) in unanesthetized, freely-moving mice. Then, we analyzed basal CBVF and S O2, and CVR of 5-month-old C57BL/6 mice during hypercapnia as well as during basic behavior such as grooming, walking and running. Moreover, we analyzed the CVR of freely-moving AD mice and their wildtype (WT) littermates during hypercapnia and could find impaired CVR in AD mice compared to WT littermates. Our results suggest that this optomechanical approach to reproducibly getting light into the brain enabled us to successfully measure CVR in unanesthetized freely-moving mice and to find impaired CVR in a mouse model of AD.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Gareau, RochaKim, Choudhury, Bamkole, Snuderl, Zou, Yaroslavsky, Jacques, Strickland, Krueger and Ahn.)
Databáze: MEDLINE
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