| Autor: |
Minkoff BB; Center for Genomic Science Innovation, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States., Burch HL; Center for Genomic Science Innovation, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States., Wolfer JD; Center for Genomic Science Innovation, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States., Sussman MR; Center for Genomic Science Innovation, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States.; Department of Biochemistry, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States. |
| Jazyk: |
angličtina |
| Zdroj: |
ACS chemical biology [ACS Chem Biol] 2023 Aug 18; Vol. 18 (8), pp. 1786-1796. Date of Electronic Publication: 2023 Jul 18. |
| DOI: |
10.1021/acschembio.3c00224 |
| Abstrakt: |
Hydrophobic microdomains, also known as hydrophobic patches, are essential for many important biological functions of water-soluble proteins. These include ligand or substrate binding, protein-protein interactions, proper folding after translation, and aggregation during denaturation. Unlike transmembrane domains, which are easily recognized from stretches of contiguous hydrophobic sidechains in amino acids via primary protein sequence, these three-dimensional hydrophobic patches cannot be easily predicted. The lack of experimental strategies for directly determining their locations hinders further understanding of their structure and function. Here, we posit that the small triatomic anion N 3 - (azide) is attracted to these patches and, in the presence of an oxidant, forms a radical that covalently modifies C-H bonds of nearby amino acids. Using two model proteins (BSA and lysozyme) and a cell-free lysate from the model higher plant Arabidopsis thaliana , we find that radical-mediated covalent azidylation occurs within buried catalytic active sites and ligand binding sites and exhibits similar behavior to established hydrophobic probes. The results herein suggest a model in which the azido radical is acting as an "affinity reagent" for nonaqueous three-dimensional protein microenvironments and is consistent with both the nonlocalized electron density of the azide moiety and the known high reactivity of azido radicals widely used in organic chemistry syntheses. We propose that the azido radical is a facile means of identifying hydrophobic microenvironments in soluble proteins and, in addition, provides a simple new method for attaching chemical handles to proteins without the need for genetic manipulation or specialized reagents. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
