Transcriptomes of human primary skin fibroblasts of healthy individuals reveal age-associated mRNAs and long noncoding RNAs.

Autor: Tsitsipatis D; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Martindale JL; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Mazan-Mamczarz K; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Herman AB; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Piao Y; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Banskota N; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Yang JH; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Cui L; Translational Gerontology Branch, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Anerillas C; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Chang MW; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Kaileh M; Laboratory of Molecular Biology and Immunology, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Munk R; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Yang X; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Ubaida-Mohien C; Translational Gerontology Branch, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Chia CW; Clinical Research Core, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Karikkineth AC; Clinical Research Core, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Zukley L; Clinical Research Core, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., D'Agostino J; Clinical Research Core, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Abdelmohsen K; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Basisty N; Translational Gerontology Branch, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., De S; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Ferrucci L; Translational Gerontology Branch, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA., Gorospe M; Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA.
Jazyk: angličtina
Zdroj: Aging cell [Aging Cell] 2023 Nov; Vol. 22 (11), pp. e13915. Date of Electronic Publication: 2023 Jul 18.
DOI: 10.1111/acel.13915
Abstrakt: Changes in the transcriptomes of human tissues with advancing age are poorly cataloged. Here, we sought to identify the coding and long noncoding RNAs present in cultured primary skin fibroblasts collected from 82 healthy individuals across a wide age spectrum (22-89 years old) who participated in the GESTALT (Genetic and Epigenetic Signatures of Translational Aging Laboratory Testing) study of the National Institute on Aging, NIH. Using high-throughput RNA sequencing and a linear regression model, we identified 1437 coding RNAs (mRNAs) and 1177 linear and circular long noncoding (lncRNAs) that were differentially abundant as a function of age. Gene set enrichment analysis (GSEA) revealed select transcription factors implicated in coordinating the transcription of subsets of differentially abundant mRNAs, while long noncoding RNA enrichment analysis (LncSEA) identified RNA-binding proteins predicted to participate in the age-associated lncRNA profiles. In summary, we report age-associated changes in the global transcriptome, coding and noncoding, from healthy human skin fibroblasts and propose that these transcripts may serve as biomarkers and therapeutic targets in aging skin.
(Published 2023. This article is a U.S. Government work and is in the public domain in the USA. Aging Cell published by Anatomical Society and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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