Arousal state transitions occlude sensory-evoked neurovascular coupling in neonatal mice.

Autor: Gheres KW; Molecular Cellular and Integrative Bioscience program, The Pennsylvania State University, University Park, PA, 16802, USA., Ünsal HS; Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.; Department of Electrical and Electronics Engineering, Abdullah Gul University, Kayseri, Türkiye., Han X; Molecular Cellular and Integrative Bioscience program, The Pennsylvania State University, University Park, PA, 16802, USA., Zhang Q; Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA, 16802, USA., Turner KL; Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA., Zhang N; Molecular Cellular and Integrative Bioscience program, The Pennsylvania State University, University Park, PA, 16802, USA.; Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.; Center for Neural Engineering, The Pennsylvania State University, University Park, PA, 16802, USA.; Center for Neurotechnology in Mental Health Research, The Pennsylvania State University, University Park, PA, 16802, USA., Drew PJ; Molecular Cellular and Integrative Bioscience program, The Pennsylvania State University, University Park, PA, 16802, USA. pjd17@psu.edu.; Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. pjd17@psu.edu.; Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA, 16802, USA. pjd17@psu.edu.; Center for Neural Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. pjd17@psu.edu.; Center for Neurotechnology in Mental Health Research, The Pennsylvania State University, University Park, PA, 16802, USA. pjd17@psu.edu.; Departments of Neurosurgery and Biology, The Pennsylvania State University, University Park, PA, 16802, USA. pjd17@psu.edu.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2023 Jul 17; Vol. 6 (1), pp. 738. Date of Electronic Publication: 2023 Jul 17.
DOI: 10.1038/s42003-023-05121-5
Abstrakt: In the adult sensory cortex, increases in neural activity elicited by sensory stimulation usually drive vasodilation mediated by neurovascular coupling. However, whether neurovascular coupling is the same in neonatal animals as adults is controversial, as both canonical and inverted responses have been observed. We investigated the nature of neurovascular coupling in unanesthetized neonatal mice using optical imaging, electrophysiology, and BOLD fMRI. We find in neonatal (postnatal day 15, P15) mice, sensory stimulation induces a small increase in blood volume/BOLD signal, often followed by a large decrease in blood volume. An examination of arousal state of the mice revealed that neonatal mice were asleep a substantial fraction of the time, and that stimulation caused the animal to awaken. As cortical blood volume is much higher during REM and NREM sleep than the awake state, awakening occludes any sensory-evoked neurovascular coupling. When neonatal mice are stimulated during an awake period, they showed relatively normal (but slowed) neurovascular coupling, showing that that the typically observed constriction is due to arousal state changes. These result show that sleep-related vascular changes dominate over any sensory-evoked changes, and hemodynamic measures need to be considered in the context of arousal state changes.
(© 2023. The Author(s).)
Databáze: MEDLINE
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