Metabolic profiling of Alzheimer's disease: Untargeted metabolomics analysis of plasma samples.

Autor: Milos T; Division of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia. Electronic address: tina.milos@irb.hr., Rojo D; Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities Madrid, Spain. Electronic address: david.rojoblanco@ceu.es., Nedic Erjavec G; Division of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia. Electronic address: gnedic@irb.hr., Konjevod M; Division of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia. Electronic address: mkonjev@irb.hr., Tudor L; Division of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia. Electronic address: ltudor@irb.hr., Vuic B; Division of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia. Electronic address: barbara.vuic@irb.hr., Svob Strac D; Division of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia. Electronic address: dsvob@irb.hr., Uzun S; School of Medicine, University of Zagreb, Zagreb, Croatia; Department for Biological Psychiatry and Psychogeriatrics, University Psychiatric Hospital Vrapče, Zagreb, Croatia. Electronic address: suzana.uzun@bolnica-vrapce.hr., Mimica N; Department for Biological Psychiatry and Psychogeriatrics, University Psychiatric Hospital Vrapče, Zagreb, Croatia. Electronic address: ninoslav.mimica@bolnica-vrapce.hr., Kozumplik O; Department for Biological Psychiatry and Psychogeriatrics, University Psychiatric Hospital Vrapče, Zagreb, Croatia. Electronic address: oliver.kozumplik@bolnica-vrapce.hr., Barbas C; Centro de Metabolómica y Bioanálisis (CEMBIO), Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities Madrid, Spain. Electronic address: cbarbas@ceu.es., Zarkovic N; Division of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia. Electronic address: zarkovic@irb.hr., Pivac N; Division of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia; University of Applied Sciences Hrvatsko Zagorje Krapina, Krapina, Croatia. Electronic address: npivac@irb.hr., Nikolac Perkovic M; Division of Molecular Medicine, Ruder Boskovic Institute, Zagreb, Croatia. Electronic address: mnikolac@irb.hr.
Jazyk: angličtina
Zdroj: Progress in neuro-psychopharmacology & biological psychiatry [Prog Neuropsychopharmacol Biol Psychiatry] 2023 Dec 20; Vol. 127, pp. 110830. Date of Electronic Publication: 2023 Jul 16.
DOI: 10.1016/j.pnpbp.2023.110830
Abstrakt: Alzheimer's disease (AD) is often not recognized or is diagnosed very late, which significantly reduces the effectiveness of available pharmacological treatments. Metabolomic analyzes have great potential for improving existing knowledge about the pathogenesis and etiology of AD and represent a novel approach towards discovering biomarkers that could be used for diagnosis, prognosis, and therapy monitoring. In this study, we applied the untargeted metabolomic approach to investigate the changes in biochemical pathways related to AD pathology. We used gas chromatography and liquid chromatography coupled to mass spectrometry (GC-MS and LC-MS, respectively) to identify metabolites whose levels have changed in subjects with AD diagnosis (N = 40) compared to healthy controls (N = 40) and individuals with mild cognitive impairment (MCI, N = 40). The GC-MS identified significant differences between groups in levels of metabolites belonging to the classes of benzene and substituted derivatives, carboxylic acids and derivatives, fatty acyls, hydroxy acids and derivatives, keto acids and derivatives, and organooxygen compounds. Most of the compounds identified by the LC-MS were various fatty acyls, glycerolipids and glycerophospholipids. All of these compounds were decreased in AD patients and in subjects with MCI compared to healthy controls. The results of the study indicate disturbed metabolism of lipids and amino acids and an imbalance of metabolites involved in energy metabolism in individuals diagnosed with AD, compared to healthy controls and MCI subjects.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests. Dubravka Svob Strac reports financial support was provided by Croatian Science Foundation. Neven Zarkovic and Coral Barbas report financial support provided by Patria Oyj.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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