Synergistic catalysis and detection of hydrogen peroxide based on a 3D-dimensional molybdenum disulfide interspersed carbon nanotubes nanonetwork immobilized chloroperoxidase biosensor.

Autor: Zhu X; School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710119, PR China. Electronic address: zhuxuefang@snnu.edu.cn., He M; School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710119, PR China. Electronic address: 13609116033@163.com., Zhang J; School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710119, PR China. Electronic address: zhangjing8902@snnu.edu.cn., Jiang Y; School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710119, PR China. Electronic address: jyc@snnu.edu.cn.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2023 Dec; Vol. 154, pp. 108507. Date of Electronic Publication: 2023 Jul 10.
DOI: 10.1016/j.bioelechem.2023.108507
Abstrakt: Enzyme-based electrochemical biosensors are promising for a wide range of applications due to their unique specificity and high sensitivity. In this work, we present a novel enzyme bioelectrode for the sensing of hydrogen peroxide (H 2 O 2 ). The molybdenum disulfide nanoflowers (MoS 2 ) is self-assembled on carboxylated carbon nanotubes (CNT) to form a three-dimensional conductive network (3D-CNT@MoS 2 ), which is modified with 1-ethyl-3-methylimidazolium bromide (IL EMB ), and followed by anchoring chloroperoxidase (CPO) onto the nanocomposite (3D-CNT@MoS 2 /IL EMB ) through covalent binding to form a bioconjugate (3D-CNT@MoS 2 /IL EMB /CPO). The IL EMB modified 3D-CNT@MoS 2 /IL EMB has good hydrophilicity and conductivity, which not only provides a suitable microenvironment for the immobilization of CPO but also facilitates the direct electron transfer (DET) of CPO at the electrode. The 3D-CNT@MoS 2 /IL EMB /CPO bioconjugate modified electrode has a high catalytic efficiency for H 2 O 2 . Through electroenzymatic synergistic catalysis for H 2 O 2 detection by 3D-CNT@MoS 2 /IL EMB /CPO-GCE, a wide detection range of 0.2 μmol·L -1 to 997 μmol·L -1 and a low detection limit of 0.097 μmol･L -1 with high sensitivity of 1050 µA·mmol·L -1 ·cm -2 were achieved. Additionally, the 3D-CNT@MoS 2 /IL EMB /CPO-GCE displayed exceptional stability, selectivity, and reproducibility. Furthermore, 3D-CNT@MoS 2 /IL EMB /CPO-GCE is suitable for sensing of H 2 O 2 in human urine s with good recovery, suggesting its potential application for the detection of H 2 O 2 in biomedical field.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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