Eco-Friendly UV-Spectrophotometric Methods Employing Magnetic Nano-Composite Polymer for the Extraction and Analysis of Sexual Boosters in Adulterated Food Products: Application of Computer-Aided Design.

Autor: Abdelshakour MA; Sohag University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Sohag 82524, Egypt., Attala K; Egyptian Russian University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Badr City, Cairo 11829, Egypt., Elsonbaty A; Egyptian Russian University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Badr City, Cairo 11829, Egypt., Abdel Salam RA; Suez Canal University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Ismailia 41522, Egypt., Hadad GM; Suez Canal University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Ismailia 41522, Egypt., Mostafa AE; Suez Canal University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, Ismailia 41522, Egypt., Eissa MS; Egyptian Russian University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Badr City, Cairo 11829, Egypt.
Jazyk: angličtina
Zdroj: Journal of AOAC International [J AOAC Int] 2023 Nov 02; Vol. 106 (6), pp. 1608-1619.
DOI: 10.1093/jaoacint/qsad084
Abstrakt: Background: Solid phase extraction (SPE) techniques, based on computationally designed magnetic-based multi-targeting molecular imprinted polymer (MT-MIP), combined with UV spectrophotometric approaches provide advantages in the examination of counterfeit samples.
Objective: The current work describes an innovative and sustainable methodology for the simultaneous determination of tadalafil (TAD) and dapoxetine hydrochloride (DAP) in aphrodisiac counterfeit products (honey and instant coffee) utilizing SPE exploiting MT-MIP. Additionally, an innovative UV spectrophotometric method capable of resolving TAD in its pharmaceutical binary mixtures with DAP was developed. A novel computational approach was implemented to tailor the synthesis and design of the MT-MIP particles.
Methods: We applied a newly developed UV spectrophotometric method which was based on a Fourier self-deconvolution (FSD) method coupled with the isoabsorptive point for determination of TAD and DAP in pharmaceutical dosage form. We also applied an SPE process based on MT-MIP designed particles, assisting in the analysis of both drugs in counterfeit food samples. The SPE process and the UV spectroscopic methodology were assessed regarding their greenness using the pioneering green analytical procedure index (GAPI), analytical greeness including sample preparation (AGREEprep) and AGREE tools. The synthesized MT-MIP particles were characterized by scanning electron microscopy and energy-dispersive x-ray spectroscopy.
Results: The suggested spectrophotometric methods revealed a wide linear concentration range of 2-50 µg/mL with lower LODs in the range of 0.604-0.994 µg/mL. Additionally, the suggested method demonstrated the utmost sensitivity and eco-friendliness for their target in its mixed dosage form and counterfeit food products.
Conclusion: The SPE process and the developed analytical UV spectroscopic methodology were validated as per the ICH guidelines, and were found to be suitable for overseeing some counterfeiting activities in commercially available honey and instant coffee aphrodisiac products.
Highlights: An SPE method based on MT-MIP magnetic-based polymer and a UV spectroscopic method were successfully developed for analysis of TAD and DAP in different matrices.
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Databáze: MEDLINE