The AUX1-AFB1-CNGC14 module establishes a longitudinal root surface pH profile.

Autor:	Serre NBC; Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic., Wernerová D; Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.; Institute of Cell and Interaction Biology, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany., Vittal P; Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic., Dubey SM; Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic., Medvecká E; Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic., Jelínková A; Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic., Petrášek J; Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.; Institute of Experimental Botany, Czech Academy of Sciences, Prague, Czech Republic., Grossmann G; Institute of Cell and Interaction Biology, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.; CEPLAS - Cluster of Excellence on Plant Sciences, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany., Fendrych M; Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2023 Jul 14; Vol. 12. Date of Electronic Publication: 2023 Jul 14.
DOI:	10.7554/eLife.85193
Abstrakt:	Plant roots navigate in the soil environment following the gravity vector. Cell divisions in the meristem and rapid cell growth in the elongation zone propel the root tips through the soil. Actively elongating cells acidify their apoplast to enable cell wall extension by the activity of plasma membrane AHA H + -ATPases. The phytohormone auxin, central regulator of gravitropic response and root development, inhibits root cell growth, likely by rising the pH of the apoplast. However, the role of auxin in the regulation of the apoplastic pH gradient along the root tip is unclear. Here, we show, by using an improved method for visualization and quantification of root surface pH, that the Arabidopsis thaliana root surface pH shows distinct acidic and alkaline zones, which are not primarily determined by the activity of AHA H + -ATPases. Instead, the distinct domain of alkaline pH in the root transition zone is controlled by a rapid auxin response module, consisting of the AUX1 auxin influx carrier, the AFB1 auxin co-receptor, and the CNCG14 calcium channel. We demonstrate that the rapid auxin response pathway is required for an efficient navigation of the root tip. Competing Interests: NS, DW, PV, SD, EM, AJ, JP, GG, MF No competing interests declared (© 2023, Serre, Wernerová et al.)
Databáze:	MEDLINE
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