Exploring the effect of utilising organic acid solutions in ultrasound-assisted extraction of pectin from apple pomace, and its potential for biomedical purposes.
| Autor: | Girón-Hernández J; Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, NE1 8ST Newcastle upon Tyne, UK., Pazmino M; Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, NE1 8ST Newcastle upon Tyne, UK., Barrios-Rodríguez YF; Department of Food Technology, Universitat Politècnica de València, Camino de Vera s/n, 46021 Valencia, Spain.; Centro Surcolombiano de Investigación en Café (CESURCAFÉ), Universidad Surcolombiana, 410007 Neiva, Colombia., Turo CT; Department of Mechanical and Aerospace Engineering, Politecnico di Torino, 10129 Turin, Italy., Wills C; School of Natural and Environmental Sciences, Newcastle University, NE1 7RU Newcastle upon Tyne, UK., Cucinotta F; School of Natural and Environmental Sciences, Newcastle University, NE1 7RU Newcastle upon Tyne, UK., Benlloch-Tinoco M; Department of Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, NE1 8ST Newcastle upon Tyne, UK., Gentile P; School of Engineering, Newcastle University, NE1 7RU Newcastle upon Tyne, UK. |
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| Jazyk: | angličtina |
| Zdroj: | Heliyon [Heliyon] 2023 Jun 28; Vol. 9 (7), pp. e17736. Date of Electronic Publication: 2023 Jun 28 (Print Publication: 2023). |
| DOI: | 10.1016/j.heliyon.2023.e17736 |
| Abstrakt: | Biomass resulting from food production represents valuable material to recover different biomolecules. In our study, we used apple pomace to obtain pectin, which is traditionally extracted using mineral acids. Our hypothesis consisted of carrying out extractions with organic acids, assisted by ultrasound, by varying processing parameters including time, temperature, and type of acid. The analytical determinations of galacturonic acid content, methoxylation and esterification degree, ζ-potential and extraction yield were used as pectin quality indicators. Pectins extracted using treatment conditions with better performance were assessed biologically in vitro for their potential to be used in biomedical applications. Overall, the extracted pectin presented a galacturonic acid content, methoxylation and esterification degree ranged from 19.7 to 67%, 26.8-41.4% and 58-65.2% respectively, and were negatively charged (-24.1 to -13.2 mV). It was found that factors of time and temperature greatly influenced the response variables excepting the esterification degree, while the acid type influenced the ζ-potential, methoxylation and esterification degrees. Additionally, it was seen that the longer extraction time (50 min) and higher temperature (50 ° C) exhibited the better extraction yield (∼10.9%). Finally, the selected pectin showed high cytocompatibility up to 500 μg/mL of concentration when seeded with Neonatal Normal Human Dermal Fibroblasts. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2023 The Authors. Published by Elsevier Ltd.) |
| Databáze: | MEDLINE |
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