Serological and Bacteriological Surveillance of Glanders Among Horses in Central Region of Iran.

Autor: Dehghan Rahimabadi P; Department of Clinical Sciences, Karaj Branch, Islamic Azad University, Karaj, Iran., Nazari A; Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran., Kamyabi M; Department of Animal Sciences, College of Agriculture and Natural Resources, University of Delaware, Newark, DE., Mosavari N; Department of Tuberculin and Mallein, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. Electronic address: nmosavari@gmail.com.
Jazyk: angličtina
Zdroj: Journal of equine veterinary science [J Equine Vet Sci] 2023 Aug; Vol. 127, pp. 104535. Date of Electronic Publication: 2023 Apr 30.
DOI: 10.1016/j.jevs.2023.104535
Abstrakt: Glanders is the oldest and very contagious disease among horses caused by Burkholderia mallei. The disease occurs as a chronic form in horses. Hence, because of the prolonged shedding, numerous horses can potentially get infected by one horse with glanders. Glanders is endemic in Iran and this causes occasional occurrence in horse population of the country. The aim of this study was to determine the incidence of B.mallei infection in horses in two central provinces of Iran. A total of 517 serum samples were collected from stable horses in Tehran and Alborz provinces. Among the studied horses, seven presented fever, anorexia, dyspnea, subcutaneous abscesses, nasal and cutaneous discharges, emaciation, and lymphadenopathy. Nasal and ocular discharges and subcutaneous abscesses were sampled for bacterial culture and PCR. The sera were examined by means of complement fixation test (CFT) and indirect enzyme-linked immunosorbent assay (iELISA). Seropositive cases were further examined by Mallein test. The results derived from the present study indicated that only 1.35% of the studied horses were positive in CFT, iELISA and Mallein test, of which only in 42.85% B.mallei was successfully cultured on blood agar and glycerinated nutrient media and confirmed by PCR. Periodic serological tests along with quarantine can benefit reduction of the occurrence of the disease in horses in Iran.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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