| Autor: |
Lakhdari W; National Institute of Agronomic Research of Algeria, Touggourt 30200, Algeria.; Valcore Laboratory, Biology Department, Faculty of Life and Nature Sciences, University of Boumerdes, Boumerdes 35000, Algeria., Benyahia I; Laboratory of Biogeochemistry and Desert Environments, Department of Chemistry, Faculty of Mathematics and Material Sciences, University of Kasdi Merbah, Ouargla 30000, Algeria., Bouhenna MM; Scientific and Technical Center of Research in Physical and Chemical Analysis (CRAPC), Bou-Ismail 42004, Algeria., Bendif H; Department of Natural and Life Sciences, Faculty of Science, University of M'sila, M'sila 28000, Algeria., Khelafi H; Valcore Laboratory, Biology Department, Faculty of Life and Nature Sciences, University of Boumerdes, Boumerdes 35000, Algeria., Bachir H; Division of Hydraulic and Bioclimatology, National Institute of Agronomic Research (INRA), Algers 16000, Algeria., Ladjal A; Valcore Laboratory, Biology Department, Faculty of Life and Nature Sciences, University of Boumerdes, Boumerdes 35000, Algeria., Hammi H; National Institute of Agronomic Research of Algeria, Touggourt 30200, Algeria., Mouhoubi D; SARL SINAL, Oran 31000, Algeria., Khelil H; SARL SINAL, Oran 31000, Algeria., Alomar TS; Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84427, Riyadh 11671, Saudi Arabia., AlMasoud N; Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84427, Riyadh 11671, Saudi Arabia., Boufafa N; SARL SINAL, Oran 31000, Algeria., Boufahja F; Biology Department, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh 11623, Saudi Arabia., Dehliz A; National Institute of Agronomic Research of Algeria, Touggourt 30200, Algeria. |
| Abstrakt: |
In this study, we investigated in vitro the potential of Trichoderma harzianum to produce bioactive secondary metabolites that can be used as alternatives to synthetic compounds. The study focused on analyzing two extracts of T. harzianum using ethyl acetate and n -butanol solvents with different polarities. The extracts were examined using phytochemical analysis to determine the content of polyphenols, flavonoids, tannins, and alkaloids. Thin-layer chromatography (TLC) and Gas chromatography-mass spectroscopy (GC-MS) analysis were used to profile volatile organic metabolites (VOCs) present in the extracts. Furthermore, the extracts were tested for their antifungal ability using the poison food technique. For measuring antioxidant activity, the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) test was used. Trichoderma harzianum was shown to have a significantly high content of tannins and alkaloids, with a noticeable difference between the two extracts. GC-MS analysis identified 33 potential compounds with numerous benefits that could be used in agriculture and the medicinal industry. Moreover, strong antifungal activity was identified against Sclerotinia sclerotiorum by 94.44%, Alternaria sp. by 77.04%, and Fusarium solani by 51.48; similarly, the IC 50 of antioxidant activity was estimated for ethyl acetate extract by 71.47% and n -butanol extract by 56.01%. This leads to the conclusion that Trichoderma harzianum VOCs play a significant role as an antifungal and antioxidant agent when taking into account the advantageous bioactive chemicals noted in the extracts. However, to our knowledge, this is the first study in Algeria presenting detailed phytochemical analysis and GC-MS profiling of Trichoderma harzianum for two extracts, ethyl acetate and n -butanol. |
