| Autor: |
Meneses CCF; Laboratório de Planejamento e Desenvolvimento de Fármacos, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belem 66075-110, Pará, Brazil., de Sousa PRM; Laboratório de Planejamento e Desenvolvimento de Fármacos, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belem 66075-110, Pará, Brazil., Lima KCN; Microbiology Laboratory, Faculty of Pharmacy, Federal University of Pará, Belem 66075-110, Pará, Brazil., Souza LMMA; Microbiology Laboratory, Faculty of Pharmacy, Federal University of Pará, Belem 66075-110, Pará, Brazil., Feio WP; Programa de Pós-Graduação em Física, Federal University of Pará, Belem 66075-110, Pará, Brazil., Remédios CMR; Programa de Pós-Graduação em Física, Federal University of Pará, Belem 66075-110, Pará, Brazil., Jouin J; Laboratoire IRCER, Université de Limoges-CNRS UMR 7315, Centre Européen de la Céramique, 87068 Limoges, France., Thomas P; Laboratoire IRCER, Université de Limoges-CNRS UMR 7315, Centre Européen de la Céramique, 87068 Limoges, France., Masson O; Laboratoire IRCER, Université de Limoges-CNRS UMR 7315, Centre Européen de la Céramique, 87068 Limoges, France., Alves CN; Laboratório de Planejamento e Desenvolvimento de Fármacos, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belem 66075-110, Pará, Brazil., Lameira J; Laboratório de Planejamento e Desenvolvimento de Fármacos, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belem 66075-110, Pará, Brazil., Monteiro MC; Microbiology Laboratory, Faculty of Pharmacy, Federal University of Pará, Belem 66075-110, Pará, Brazil. |
| Abstrakt: |
Caffeic acid (CA) exhibits a myriad of biological activities including cardioprotective action, antioxidant, antitumor, anti-inflammatory, and antimicrobial properties. On the other hand, CA presents low water solubility and poor bioavailability, which have limited its use for therapeutic applications. The objective of this study was to develop a nanohybrid of zinc basic salts (ZBS) and chitosan (Ch) containing CA (ZBS-CA/Ch) and evaluate its anti-edematogenic and antioxidant activity in dextran and carrageenan-induced paw edema model. The samples were obtained by coprecipitation method and characterized by X-ray diffraction, Fourier transform infrared (FT-IR), scanning electron microscope (SEM) and UV-visible spectroscopy. The release of caffeate anions from ZBS-CA and ZBS-CA/Ch is pH-dependent and is explained by a pseudo-second order kinetics model, with a linear correlation coefficient of R 2 ≥ 0.99 at pH 4.8 and 7.4. The in vivo pharmacological assays showed excellent anti-edematogenic and antioxidant action of the ZBS-CA/Ch nanoparticle with slowly releases of caffeate anions in the tissue, leading to a prolongation of CA-induced anti-edematogenic and anti-inflammatory activities, as well as improving its inhibition or sequestration antioxidant action toward reactive species. Overall, this study highlighted the importance of ZBS-CA/Ch as an optimal drug carrier. |
