Control of the Electroporation Efficiency of Nanosecond Pulses by Swinging the Electric Field Vector Direction.

Autor: Kim V; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA., Semenov I; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA., Kiester AS; Bioeffects Division, Airman System Directorate, 711th Human Performance Wing, Air Force Research Laboratory, JBSA Fort Sam Houston, San Antonio, TX 78234, USA., Keppler MA; SAIC, San Antonio, TX 78234, USA., Ibey BL; Bioeffects Division, Airman System Directorate, 711th Human Performance Wing, Air Force Research Laboratory, JBSA Fort Sam Houston, San Antonio, TX 78234, USA., Bixler JN; Bioeffects Division, Airman System Directorate, 711th Human Performance Wing, Air Force Research Laboratory, JBSA Fort Sam Houston, San Antonio, TX 78234, USA., Colunga Biancatelli RML; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA.; Department of Physiological Sciences, Eastern Virginia Medical School, Norfolk, VA 23508, USA., Pakhomov AG; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23508, USA.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Jun 30; Vol. 24 (13). Date of Electronic Publication: 2023 Jun 30.
DOI: 10.3390/ijms241310921
Abstrakt: Reversing the pulse polarity, i.e., changing the electric field direction by 180°, inhibits electroporation and electrostimulation by nanosecond electric pulses (nsEPs). This feature, known as "bipolar cancellation," enables selective remote targeting with nsEPs and reduces the neuromuscular side effects of ablation therapies. We analyzed the biophysical mechanisms and measured how cancellation weakens and is replaced by facilitation when nsEPs are applied from different directions at angles from 0 to 180°. Monolayers of endothelial cells were electroporated by a train of five pulses (600 ns) or five paired pulses (600 + 600 ns) applied at 1 Hz or 833 kHz. Reversing the electric field in the pairs (180° direction change) caused 2-fold (1 Hz) or 20-fold (833 kHz) weaker electroporation than the train of single nsEPs. Reducing the angle between pulse directions in the pairs weakened cancellation and replaced it with facilitation at angles <160° (1 Hz) and <130° (833 kHz). Facilitation plateaued at about three-fold stronger electroporation compared to single pulses at 90-100° angle for both nsEP frequencies. The profound dependence of the efficiency on the angle enables novel protocols for highly selective focal electroporation at one electrode in a three-electrode array while avoiding effects at the other electrodes. Nanosecond-resolution imaging of cell membrane potential was used to link the selectivity to charging kinetics by co- and counter-directional nsEPs.
Databáze: MEDLINE
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