Fighting the battle against evolution: designing genetically modified organisms for evolutionary stability.

Autor: Arbel-Groissman M; Shmunis School of Biomedicine and Cancer Research, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6997801, Israel., Menuhin-Gruman I; School of Mathematical Sciences, The Raymond and Beverly Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv 6997801, Israel., Naki D; Shmunis School of Biomedicine and Cancer Research, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 6997801, Israel., Bergman S; Department of Biomedical Engineering, Tel Aviv University, Tel Aviv 6997801, Israel., Tuller T; Department of Biomedical Engineering, Tel Aviv University, Tel Aviv 6997801, Israel; The Sagol School of Neuroscience, Tel-Aviv University, Tel Aviv 6997801, Israel. Electronic address: tamirtul@tauex.tau.ac.il.
Jazyk: angličtina
Zdroj: Trends in biotechnology [Trends Biotechnol] 2023 Dec; Vol. 41 (12), pp. 1518-1531. Date of Electronic Publication: 2023 Jul 12.
DOI: 10.1016/j.tibtech.2023.06.008
Abstrakt: Synthetic biology has made significant progress in many areas, but a major challenge that has received limited attention is the evolutionary stability of synthetic constructs made of heterologous genes. The expression of these constructs in microorganisms, that is, production of proteins that are not necessary for the organism, is a metabolic burden, leading to a decrease in relative fitness and make the synthetic constructs unstable over time. This is a significant concern for the synthetic biology community, particularly when it comes to bringing this technology out of the laboratory. In this review, we discuss the issue of evolutionary stability in synthetic biology and review the available tools to address this challenge.
Competing Interests: Declaration of interests None are declared.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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