Enhance the oral insulin delivery route using a modified chitosan-based formulation fabricated by microwave.

Autor: AlSalem HS; Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. Electronic address: husalsalem@pnu.edu.sa., Abdulsalam NM; Department of Food and Nutrition, Faculty of Human Sciences and Design, King Abdul Aziz University, P.O. Box 42807, Jeddah 21551, Saudi Arabia., Khateeb NA; Clinical Nutrition Department, College of Applied Medical Sciences-King Saud bin Abdulaziz University for Health Sciences, P.O. Box 2477. Mail Code 527, Al Ahsa 31982, Saudi Arabia., Binkadem MS; Department of Chemistry, College of Science, University of Jeddah, P.O. Box 80327, Jeddah 21589, Saudi Arabia. Electronic address: 04100507@uj.edu.sa., Alhadhrami NA; Chemistry Department, Faculty of Science, Taibah University, P.O. Box 30002, Medina 42353, Saudi Arabia. Electronic address: nhadhrami@taibahu.edu.sa., Khedr AM; Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt. Electronic address: abdallah.khader@science.tanta.edu.eg., Abdelmonem R; Department of Industrial Pharmacy, Faculty of Pharmacy, Misr University for Science & Technology, 6th October, Egypt., Shoueir KR; Institute of Nanoscience & Nanotechnology, Kafrelsheikh University, 33516 Kafrelsheikh, Egypt. Electronic address: kameltag@yahoo.com., Nadwa EH; Department of Pharmacology and Therapeutics, College of Medicine, Jouf University, Sakaka 72345, Saudi Arabia; Department of Medical Pharmacology, Faculty of Medicine, Cairo University, Giza 12613, Egypt.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Aug 30; Vol. 247, pp. 125779. Date of Electronic Publication: 2023 Jul 11.
DOI: 10.1016/j.ijbiomac.2023.125779
Abstrakt: Chitosan (Cs) was subjected to ball milling and subsequently functionalized with Dinitro salicylic acid (Cs-DNS) to enhance the efficacy of oral insulin delivery. The hydrodynamic spherical particle sizes exhibited 33.29 ± 5.08 nm for modified Cs-DNS NPs. Irrespective of insulin entrapment, zeta potential measurements revealed positively charged Cs-DNS NPs (+ 35 ± 3.5 mV). The entrapment performance (EP%) was evaluated in vitro, and insulin release patterns at various pH levels. The EP% for Cs-DNS NPs was 99.3 ± 1.6. Cs- DNS NPs retained a considerable amount of insulin (92 %) in an acidic medium, and significant quantities were released at increasing pH values over time. In vivo investigations, the diabetic rats which taken insulin-incorporated NPs had lower serum glucose levels (SGL) after 3 h to (39.4 ± 0.6 %) for Cs- DNS NPs. For insulin-incorporated Cs- DNS NPs, the bioavailability (BA%) and pharmacological availability (PA%) were 17.5 ± 0.31 % and 8.6 ± 0.8 %, respectively. The assertion above highlights the significance and effectiveness of modified chitosan in promoting insulin delivery, decreasing SGL levels, and guaranteeing safety.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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