Detection of the clinically persistent, pathogenic yeast spp. Candida auris from hospital and municipal wastewater in Miami-Dade County, Florida.

Autor: Babler K; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA., Sharkey M; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Arenas S; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Amirali A; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA., Beaver C; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Comerford S; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Goodman K; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Institute for Data Science & Computing, University of Miami, Coral Gables, FL, USA., Grills G; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Holung M; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Kobetz E; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Laine J; Environmental Health and Safety, University of Miami, Miami, FL 33136, USA., Lamar W; Division of Occupational Health, Safety & Compliance, University of Miami Health System, Miami, FL 33136, USA., Mason C; Department of Physiology and Biophysics, Weill Cornell Medical College, New York City, NY 10021, USA; The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, Weill Cornell Medicine, New York, NY 10021, USA; The WorldQuant Initiative for Quantitative Prediction, Weill Cornell Medicine, New York, NY 10021, USA., Pronty D; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Reding B; Environmental Health and Safety, University of Miami, Miami, FL 33136, USA., Schürer S; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Department of Molecular & Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Institute for Data Science & Computing, University of Miami, Coral Gables, FL, USA., Schaefer Solle N; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA; Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Stevenson M; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Vidović D; Department of Molecular & Cellular Pharmacology, University of Miami Miller School of Medicine, Miami, FL 33136, USA., Solo-Gabriele H; Department of Chemical, Environmental, and Materials Engineering, University of Miami, Coral Gables, FL 33146, USA., Shukla B; Department of Medicine, University of Miami Miller School of Medicine, Miami, FL 33136, USA. Electronic address: bxs729@miami.edu.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2023 Nov 10; Vol. 898, pp. 165459. Date of Electronic Publication: 2023 Jul 12.
DOI: 10.1016/j.scitotenv.2023.165459
Abstrakt: The use of wastewater-based surveillance (WBS) for detecting pathogens within communities has been growing since the beginning of the COVID-19 pandemic with early efforts investigating severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA in wastewater. Recent efforts have shed light on the utilization of WBS for alternative targets, such as fungal pathogens, like Candida auris, in efforts to expand the technology to assess non-viral targets. The objective of this study was to extend workflows developed for SARS-CoV-2 quantification to evaluate whether C. auris can be recovered from wastewater, inclusive of effluent from a wastewater treatment plant (WWTP) and from a hospital with known numbers of patients colonized with C. auris. Measurements of C. auris in wastewater focused on culture-based methods and quantitative PCR (qPCR). Results showed that C. auris can be cultured from wastewater and that levels detected by qPCR were higher in the hospital wastewater compared to the wastewater from the WWTP, suggesting either dilution or degradation of this pathogenic yeast at downstream collection points. The results from this study illustrate that WBS can extend beyond SARS-CoV-2 monitoring to evaluate additional non-viral pathogenic targets and demonstrates that C. auris isolated from wastewater is competent to replicate in vitro using fungal-specific culture media.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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