Fabrication of Insoluble Elastin by Enzyme-Free Cross-Linking.

Autor: Hedtke T; Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, 06120, Halle (Saale), Germany.; Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg, Halle (Saale), 06120, Halle (Saale), Germany., Mende M; Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg, Halle (Saale), 06120, Halle (Saale), Germany., Steenbock H; Institute of Virology and Cell Biology, University of Lübeck, 23562, Lübeck, Germany., Brinckmann J; Institute of Virology and Cell Biology, University of Lübeck, 23562, Lübeck, Germany.; Department of Dermatology, University of Lübeck, 23538, Lübeck, Germany., Menzel M; Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, 06120, Halle (Saale), Germany., Hoehenwarter W; Proteome Analytics Research Group, Leibniz Institute for Plant Biochemistry, 06120, Halle (Saale), Germany., Pietzsch M; Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg, Halle (Saale), 06120, Halle (Saale), Germany.; Institute of Applied Dermatopharmacy at the Martin Luther University Halle-Wittenberg (IADP), 06120, Halle (Saale), Germany., Groth T; Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg, Halle (Saale), 06120, Halle (Saale), Germany.; Interdisciplinary Center of Materials Science, Martin Luther University Halle-Wittenberg, 06120, Halle (Saale), Germany., Schmelzer CEH; Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, 06120, Halle (Saale), Germany.; Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg, Halle (Saale), 06120, Halle (Saale), Germany.; Institute of Applied Dermatopharmacy at the Martin Luther University Halle-Wittenberg (IADP), 06120, Halle (Saale), Germany.
Jazyk: angličtina
Zdroj: Macromolecular bioscience [Macromol Biosci] 2023 Nov; Vol. 23 (11), pp. e2300203. Date of Electronic Publication: 2023 Jul 19.
DOI: 10.1002/mabi.202300203
Abstrakt: Elastin is an essential extracellular matrix protein that enables tissues and organs such as arteries, lungs, and skin, which undergo continuous deformation, to stretch and recoil. Here, an approach to fabricating artificial elastin with close-to-native molecular and mechanical characteristics is described. Recombinantly produced tropoelastin are polymerized through coacervation and allysine-mediated cross-linking induced by pyrroloquinoline quinone (PQQ). A technique that allows the recovery and repeated use of PQQ for protein cross-linking by covalent attachment to magnetic Sepharose beads is developed. The produced material closely resembles natural elastin in its molecular, biochemical, and mechanical properties, enabled by the occurrence of the cross-linking amino acids desmosine, isodesmosine, and merodesmosine. It possesses elevated resistance against tryptic proteolysis, and its Young's modulus ranging between 1 and 2 MPa is similar to that of natural elastin. The approach described herein enables the engineering of mechanically resilient, elastin-like materials for biomedical applications.
(© 2023 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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