Characterization of cysteine proteases from poultry red mite, tropical fowl mite, and northern fowl mite to assess the feasibility of developing a broadly efficacious vaccine against multiple mite species.

Autor: Win SY; Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan., Murata S; Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan.; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan., Fujisawa S; Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan., Seo H; Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan., Sato J; Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan., Motai Y; Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan., Sato T; Vaxxinova Japan K.K., Minato-ku, Tokyo, Japan., Oishi E; Vaxxinova Japan K.K., Minato-ku, Tokyo, Japan., Taneno A; Vaxxinova Japan K.K., Minato-ku, Tokyo, Japan., Htun LL; Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw, Myanmar., Bawm S; Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw, Myanmar.; Department of Livestock and Aquaculture Research, Ministry of Agriculture, Livestock and Irrigation, Nay Pyi Taw, Myanmar., Okagawa T; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan., Maekawa N; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan., Konnai S; Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan.; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan., Ohashi K; Laboratory of Infectious Diseases, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan.; Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan.; International Affairs Office, Faculty of Veterinary Medicine, Hokkaido University, Kita-ku, Sapporo, Japan.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Jul 13; Vol. 18 (7), pp. e0288565. Date of Electronic Publication: 2023 Jul 13 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0288565
Abstrakt: Infestation with poultry red mites (PRM, Dermanyssus gallinae) causes anemia, reduced egg production, and death in serious cases, resulting in significant economic losses to the poultry industry. As a novel strategy for controlling PRMs, vaccine approaches have been focused upon and several candidate vaccine antigens against PRMs have been reported. Tropical (TFM, Ornithonyssus bursa) and northern (NFM, Ornithonyssus sylviarum) fowl mites are also hematophagous and cause poultry industry problems similar to those caused by PRM. Therefore, ideal antigens for anti-PRM vaccines are molecules that cross-react with TFMs and NFMs, producing pesticidal effects similar to those against PRMs. In this study, to investigate the potential feasibility of developing vaccines with broad efficacy across mite species, we identified and characterized cysteine proteases (CPs) of TFMs and NFMs, which were previously reported to be effective vaccine antigens of PRMs. The open reading frames of CPs from TFMs and NFMs had the same sequences, which was 73.0% similar to that of PRMs. Phylogenetic analysis revealed that the CPs of TFMs and NFMs clustered in the same clade as CPs of PRMs. To assess protein functionality, we generated recombinant peptidase domains of CPs (rCP-PDs), revealing all rCP-PDs showed CP-like activities. Importantly, the plasma obtained from chickens immunized with each rCP-PD cross-reacted with rCP-PDs of different mites. Finally, all immune plasma of rCP-PDs reduced the survival rate of PRMs, even when the plasma was collected from chickens immunized with rCP-PDs derived from TFM and NFM. Therefore, CP antigen is a promising, broadly efficacious vaccine candidate against different avian mites.
Competing Interests: SM, SK, and KO are authors of a patent covering the materials and techniques described in this manuscript (EU patent EP15800403.6; Japanese patent, 2016-523581). TS, EO, and AT are employed by Vaxxinova Japan K. K., Tokyo, Japan. All other authors have no competing interests to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
(Copyright: © 2023 Win et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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