| Autor: |
Chew KL; Department of Laboratory Medicine, National University Hospital, Singapore, Singapore., Achik R; Department of Laboratory Medicine, National University Hospital, Singapore, Singapore., Osman NH; Environmental Health Institute, National Environment Agency, Singapore, Singapore., Octavia S; Environmental Health Institute, National Environment Agency, Singapore, Singapore., Teo JWP; Department of Laboratory Medicine, National University Hospital, Singapore, Singapore. |
| Jazyk: |
angličtina |
| Zdroj: |
Microbial genomics [Microb Genom] 2023 Jul; Vol. 9 (7). |
| DOI: |
10.1099/mgen.0.001047 |
| Abstrakt: |
Invasive candida infections are significant infections that may occur in vulnerable patients with high rates of mortality or morbidity. Drug-resistance rates also appear to be on the rise which further complicate treatment options and outcomes. The aims of this study were to describe the prevalence, molecular epidemiology, and genetic features of Candida bloodstream isolates in a hospital setting. The resistance mechanisms towards the two most commonly administered antifungals, fluconazole and anidulafungin, were determined. Blood culture isolates between 1 January 2018 and 30 June 2021 positive for Candida spp. were included. Susceptibility testing was performed using Etest. Whole-genome-sequencing was performed using Illumina NovaSeq with bioinformatics analysis performed. A total of 203 isolates were sequenced: 56 C . glabrata, 53 C . tropicalis , 44 C . albicans, 36 C . parapsilosis complex (consisting of C. parapsilosis, C. orthopsilosis, and C. metapsilosis ), six C . krusei, five C . dubliniensis , and three C . auris . A single cluster of azole-resistant C. tropicalis, and four clusters of C. parapsilosis isolates were observed, suggesting possible transmission occurring over several years. We found 11.3%, and 52.7 % of C. tropicalis and C. parapsilosis , respectively, clustered with other isolates, suggesting exogenous sources may play a significant role of transmission, particularly for C. parapsilosis . The clusters spanned over several years suggesting the possibility of environmental reservoirs contributing to the spread. Limited clonality was seen for C. albicans . Several sequence types appeared to be dominant for C. glabrata, however the SNP differences varied widely, indicating absence of sustained transmission. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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