Comparative physiological, antioxidant and proteomic investigation reveal robust response to cold stress in Digitalis purpurea L.

Autor: Jan N; Department of Botany, University of Kashmir, Hazratbal, Srinagar, 190 006, Jammu and Kashmir, India., Wani UM; Department of Botany, University of Kashmir, Hazratbal, Srinagar, 190 006, Jammu and Kashmir, India., Wani MA; Department of Botany, University of Kashmir, Hazratbal, Srinagar, 190 006, Jammu and Kashmir, India., Qazi HA; Department of Botany, University of Kashmir, Hazratbal, Srinagar, 190 006, Jammu and Kashmir, India., John R; Department of Botany, University of Kashmir, Hazratbal, Srinagar, 190 006, Jammu and Kashmir, India. riffatminhaj@kashmiruniversity.ac.in.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2023 Sep; Vol. 50 (9), pp. 7319-7331. Date of Electronic Publication: 2023 Jul 13.
DOI: 10.1007/s11033-023-08635-7
Abstrakt: Background of the Study: Digitalis purpurea (L) is an important medicinal plant growing at Alpine region of Himalayas and withstands low temperatures and harsh climatic conditions existing at high altitude. It serves as an ideal plant system to decipher the tolerance to cold stress (CS) in plants from high altitudes.
Methods and Results: To understand the complexity of plant response to CS, we performed a comparative physiological and biochemical study complemented with proteomics in one-month-old D. purpurea grown at 25 °C (control) and 4 °C (CS). We observed an enhanced accumulation of different osmo-protectants (glycine betaine, soluble sugar and proline) and higher transcription (mRNA levels) of various antioxidant enzymes with an increased antioxidant enzyme activity in D. purpurea when exposed to CS. Furthermore, higher concentrations of non-enzymatic antioxidants (flavonoids, phenolics) was also associated with the response to CS. Differential proteomic analysis revealed the role of various proteins primarily involved in redox reactions, protein stabilization, quinone and sterol metabolism involved in CS response in D. purpurea..
Conclusion: Our results provide a framework for better understanding the physiological and molecular mechanism of CS response in D. purpurea at high altitudes.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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