A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus Magnaporthe oryzae within rice cells.

Autor: Li G; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, USA., Gong Z; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, USA.; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China., Dulal N; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, USA., Marroquin-Guzman M; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, USA.; Bayer CropScience, Chesterfield, MO, USA., Rocha RO; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, USA.; Department of Plant Pathology and Ecology, The Connecticut Agricultural Experiment Station, New Haven, CT, USA., Richter M; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, USA., Wilson RA; Department of Plant Pathology, University of Nebraska-Lincoln, Lincoln, NE, USA. rwilson10@unl.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Jul 12; Vol. 14 (1), pp. 4146. Date of Electronic Publication: 2023 Jul 12.
DOI: 10.1038/s41467-023-39880-w
Abstrakt: The blast fungus Magnaporthe oryzae produces invasive hyphae in living rice cells during early infection, separated from the host cytoplasm by plant-derived interfacial membranes. However, the mechanisms underpinning this intracellular biotrophic growth phase are poorly understood. Here, we show that the M. oryzae serine/threonine protein kinase Rim15 promotes biotrophic growth by coordinating cycles of autophagy and glutaminolysis in invasive hyphae. Alongside inducing autophagy, Rim15 phosphorylates NAD-dependent glutamate dehydrogenase, resulting in increased levels of α-ketoglutarate that reactivate target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Deleting RIM15 attenuates invasive hyphal growth and triggers plant immunity; exogenous addition of α-ketoglutarate prevents these effects, while glucose addition only suppresses host defenses. Our results indicate that Rim15-dependent cycles of autophagic flux liberate α-ketoglutarate - via glutaminolysis - to reactivate TOR signaling and fuel biotrophic growth while conserving glucose for antioxidation-mediated host innate immunity suppression.
(© 2023. The Author(s).)
Databáze: MEDLINE
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