[Intranasal vaccine against COVID-19 based on a recombinant variant of the Sendai virus (Paramyxoviridae: Respirovirus ) strain Moscow].

Autor:	Kudrov GA; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Zainutdinov SS; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Grazhdantseva AA; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Shipovalov AV; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Sivolobova GF; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Semenova AV; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Merkuleva IA; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Shcherbakov DN; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Taranov OS; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Zaykovskaya AV; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Shulgina IS; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Pyankov OV; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor., Kochneva GV; State Research Center of Virology and Biotechnology 'Vector' of Rospotrebnadzor.
Jazyk:	ruština
Zdroj:	Voprosy virusologii [Vopr Virusol] 2023 Jul 06; Vol. 68 (3), pp. 215-227. Date of Electronic Publication: 2023 Jul 06.
DOI:	10.36233/0507-4088-172
Abstrakt:	Introduction: Intranasal vaccination using live vector vaccines based on non-pathogenic or slightly pathogenic viruses is the one of the most convenient, safe and effective ways to prevent respiratory infections, including COVID-19. Sendai virus is the best suited for this purpose, since it is respiratory virus and is capable of limited replication in human bronchial epithelial cells without causing disease. The aim of the work is to design and study the vaccine properties of recombinant Sendai virus, Moscow strain, expressing secreted receptor-binding domain of SARS-CoV-2 Delta strain S protein (RBDdelta) during a single intranasal immunization. Materials and Methods: Recombinant Sendai virus carrying insertion of RBDdelta transgene between P and M genes was constructed using reverse genetics and synthetic biology methods. Expression of RBDdelta was analyzed by Western blot. Vaccine properties were studied in two models: Syrian hamsters and BALB/c mice. Immunogenicity was evaluated by ELISA and virus-neutralization assays. Protectiveness was assessed by quantitation of SARS-CoV-2 RNA in RT-PCR and histological analysis of the lungs. Results: Based on Sendai virus Moscow strain, a recombinant Sen-RBDdelta(M) was constructed that expressed a secreted RBDdelta immunologically identical to natural SARS-CoV-2 protein. A single intranasal administration of Sen-RBDdelta(M) to hamsters and mice significantly, by 15 and 107 times, respectively, reduced replicative activity of SARS-CoV-2 in lungs of animals, preventing the development of pneumonia. An effective induction of virus-neutralizing antibodies has also been demonstrated in mice. Conclusion: Sen-RBDdelta(M) is a promising vaccine construct against SARS-CoV-2 infection and has a protective properties even after a single intranasal introduction.
Databáze:	MEDLINE
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