Reduced Graphene Oxide Electrolyte-Gated Transistor Immunosensor with Highly Selective Multiparametric Detection of Anti-Drug Antibodies.

Autor: Sensi M; Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, Modena, 41125, Italy., de Oliveira RF; Université de Strasbourg, CNRS, ISIS, 8 allée Gaspard Monge, Strasbourg, 67000, France.; Brazilian Nanotechnology National Laboratory (LNNano), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, 13083-970, Brazil., Berto M; Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, Modena, 41125, Italy., Palmieri M; Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, Modena, 41125, Italy., Ruini E; Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, Modena, 41125, Italy., Livio PA; Université de Strasbourg, CNRS, ISIS, 8 allée Gaspard Monge, Strasbourg, 67000, France., Conti A; Dermatology Unit, Surgical, Medical, and Dental Department of Morphological Sciences Related to Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, via del Pozzo 71, Modena, 41125, Italy., Pinti M; Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, Modena, 41125, Italy., Salvarani C; Rheumatology Unit, University of Modena and Reggio Emilia, Medical School Azienda Ospedaliero-Universitaria Policlinico di Modena, via del Pozzo 71, Modena, 41125, Italy., Cossarizza A; Department of Medical and Surgical Sciences for Children and Adults, University of Modena and Reggio Emilia, Via Campi 287, Modena, 41125, Italy., Cabot JM; Leitat Technology Center, Innovació 2, Barcelona, 08225, Spain., Ricart J; Leitat Technology Center, Innovació 2, Barcelona, 08225, Spain., Casalini S; Université de Strasbourg, CNRS, ISIS, 8 allée Gaspard Monge, Strasbourg, 67000, France.; Dipartimento di Scienze Chimiche University of Padova, via Marzolo 1, Padova, 35131, Italy., González-García MB; Metrohm DropSens, S.L. Vivero Ciencias de la Salud, C/Colegio Santo Domingo de Guzmán s/n, Oviedo, 33010, Spain., Fanjul-Bolado P; Metrohm DropSens, S.L. Vivero Ciencias de la Salud, C/Colegio Santo Domingo de Guzmán s/n, Oviedo, 33010, Spain., Bortolotti CA; Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, Modena, 41125, Italy., Samorì P; Université de Strasbourg, CNRS, ISIS, 8 allée Gaspard Monge, Strasbourg, 67000, France., Biscarini F; Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, Modena, 41125, Italy.; Center for Translational Neurophysiology, Istituto Italiano di Tecnologia, Via Fossato di Mortara 17-19, Ferrara, 44121, Italy.
Jazyk: angličtina
Zdroj: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2023 Sep; Vol. 35 (36), pp. e2211352. Date of Electronic Publication: 2023 Jul 28.
DOI: 10.1002/adma.202211352
Abstrakt: The advent of immunotherapies with biological drugs has revolutionized the treatment of cancers and auto-immune diseases. However, in some patients, the production of anti-drug antibodies (ADAs) hampers the drug efficacy. The concentration of ADAs is typically in the range of 1-10 pm; hence their immunodetection is challenging. ADAs toward Infliximab (IFX), a drug used to treat rheumatoid arthritis and other auto-immune diseases, are focussed. An ambipolar electrolyte-gated transistor (EGT) immunosensor is reported based on a reduced graphene oxide (rGO) channel and IFX bound to the gate electrode as the specific probe. The rGO-EGTs are easy to fabricate and exhibit low voltage operations (≤ 0.3 V), a robust response within 15 min, and ultra-high sensitivity (10 am limit of detection). A multiparametric analysis of the whole rGO-EGT transfer curves based on the type-I generalized extreme value distribution is proposed. It is demonstrated that it allows to selectively quantify ADAs also in the co-presence of its antagonist tumor necrosis factor alpha (TNF-α), the natural circulating target of IFX.
(© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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