Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis.

Autor: Bitzenhofer NL; Institute of Molecular Enzyme Technology (IMET), Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Höfel C; Institute of Molecular Enzyme Technology (IMET), Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Thies S; Institute of Molecular Enzyme Technology (IMET), Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Weiler AJ; Institute of Molecular Enzyme Technology (IMET), Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Eberlein C; Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany., Heipieper HJ; Department of Environmental Biotechnology, Helmholtz Centre for Environmental Research (UFZ), Leipzig, Germany., Batra-Safferling R; Institute of Biological Information Processing - Structural Biochemistry (IBI-7: Structural Biochemistry), Forschungszentrum Jülich, Jülich, Germany., Sundermeyer P; Ernst-Ruska Centre for Microscopy and Spectroscopy with Electrons (ER-C-3/Structural Biology), Forschungszentrum Jülich, Jülich, Germany.; Institute for Biological Information Processing 6 (IBI-6/ Structural Cellular Biology), Forschungszentrum Jülich, Jülich, Germany., Heidler T; Ernst-Ruska Centre for Microscopy and Spectroscopy with Electrons (ER-C-3/Structural Biology), Forschungszentrum Jülich, Jülich, Germany.; Institute for Biological Information Processing 6 (IBI-6/ Structural Cellular Biology), Forschungszentrum Jülich, Jülich, Germany., Sachse C; Ernst-Ruska Centre for Microscopy and Spectroscopy with Electrons (ER-C-3/Structural Biology), Forschungszentrum Jülich, Jülich, Germany.; Institute for Biological Information Processing 6 (IBI-6/ Structural Cellular Biology), Forschungszentrum Jülich, Jülich, Germany.; Department of Biology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Busche T; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany.; Bielefeld University, Medical School East Westphalia-Lippe, Bielefeld University, Bielefeld, Germany., Kalinowski J; Center for Biotechnology (CeBiTec), Bielefeld University, Bielefeld, Germany., Belthle T; DWI─Leibniz-Institute for Interactive Materials, Aachen, Germany.; Functional and Interactive Polymers, Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Aachen, Germany., Drepper T; Institute of Molecular Enzyme Technology (IMET), Heinrich Heine University Düsseldorf, Düsseldorf, Germany., Jaeger KE; Institute of Molecular Enzyme Technology (IMET), Heinrich Heine University Düsseldorf, Düsseldorf, Germany.; Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich, Jülich, Germany., Loeschcke A; Institute of Molecular Enzyme Technology (IMET), Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Jazyk: angličtina
Zdroj: Microbial biotechnology [Microb Biotechnol] 2024 Jan; Vol. 17 (1), pp. e14312. Date of Electronic Publication: 2023 Jul 12.
DOI: 10.1111/1751-7915.14312
Abstrakt: Pseudomonas species have become promising cell factories for the production of natural products due to their inherent robustness. Although these bacteria have naturally evolved strategies to cope with different kinds of stress, many biotechnological applications benefit from engineering of optimised chassis strains with specially adapted tolerance traits. Here, we explored the formation of outer membrane vesicles (OMV) of Pseudomonas putida KT2440. We found OMV production to correlate with the recombinant production of a natural compound with versatile beneficial properties, the tripyrrole prodigiosin. Further, several P. putida genes were identified, whose up- or down-regulated expression allowed controlling OMV formation. Finally, genetically triggering vesiculation in production strains of the different alkaloids prodigiosin, violacein, and phenazine-1-carboxylic acid, as well as the carotenoid zeaxanthin, resulted in up to three-fold increased product yields. Consequently, our findings suggest that the construction of robust strains by genetic manipulation of OMV formation might be developed into a useful tool which may contribute to improving limited biotechnological applications.
(© 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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