Sole microbiome progression in a hatchery life cycle, from egg to juvenile.

Autor:	Almeida DB; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, University of Porto, Porto, Portugal.; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Matosinhos, Portugal.; SEA EIGHT - Safiestela S.A., Estela, Portugal., Semedo M; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Matosinhos, Portugal., Magalhães C; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Matosinhos, Portugal.; FCUP - Faculty of Sciences, University of Porto, Porto, Portugal., Blanquet I; SEA EIGHT - Safiestela S.A., Estela, Portugal., Mucha AP; CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Matosinhos, Portugal.; FCUP - Faculty of Sciences, University of Porto, Porto, Portugal.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Jun 26; Vol. 14, pp. 1188876. Date of Electronic Publication: 2023 Jun 26 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1188876
Abstrakt:	Recirculating aquaculture systems (RAS) pose unique challenges in microbial community management since they rely on a stable community with key target groups, both in the RAS environment and in the host (in this case, Solea senegalensis ). Our goal was to determine how much of the sole microbiome is inherited from the egg stage, and how much is acquired during the remainder of the sole life cycle in an aquaculture production batch, especially regarding potentially probiotic and pathogenic groups. Our work comprises sole tissue samples from 2 days before hatching and up to 146 days after hatching (-2 to 146 DAH), encompassing the egg, larval, weaning, and pre-ongrowing stages. Total DNA was isolated from the different sole tissues, as well as from live feed introduced in the first stages, and 16S rRNA gene was sequenced (V6-V8 region) using the Illumina MiSeq platform. The output was analysed with the DADA2 pipeline, and taxonomic attribution with SILVAngs version 138.1. Using the Bray-Curtis dissimilarity index, both age and life cycle stage appeared to be drivers of bacterial community dissimilarity. To try to distinguish the inherited (present since the egg stage) from the acquired community (detected at later stages), different tissues were analysed at 49, 119 and 146 DAH (gill, intestine, fin and mucus). Only a few genera were inherited, but those that were inherited accompany the sole microbiome throughout the life cycle. Two genera of potentially probiotic bacteria ( Bacillus and Enterococcus ) were already present in the eggs, while others were acquired later, in particularly, forty days after live feed was introduced. The potentially pathogenic genera Tenacibaculum and Vibrio were inherited from the eggs, while Photobacterium and Mycobacterium seemed to be acquired at 49 and 119 DAH, respectively. Significant co-occurrence was found between Tenacibaculum and both Photobacterium and Vibrio . On the other hand, significantly negative correlations were detected between Vibrio and Streptococcus , Bacillus , Limosilactobacillus and Gardnerella . Our work reinforces the importance of life cycle studies, which can contribute to improve production husbandry strategies. However, we still need more information on this topic as repetition of patterns in different settings is essential to confirm our findings. Competing Interests: DA and IB were employed by Safiestela S.A., SEA EIGHT group at the time of the study. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Almeida, Semedo, Magalhães, Blanquet and Mucha.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu