p16 INK4a and pRb expression in laryngeal squamous cell carcinoma with and without infection by EBV or different genotypes of HPV: a retrospective study.

Autor: Vazquez-Guillen JM; Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico., Palacios-Saucedo GC; Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo Leon, Mexico. palsaugc@gmail.com., Alanis-Valdez AY; Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico., Huerta-Escobedo A; Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico., Zavala-Pompa A; Laboratorio Medicina Diagnostica S.A. de C.V., Monterrey, Nuevo Leon, Mexico., Rivera-Morales LG; Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico., Martinez-Torres AC; Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico., Gonzalez-Villasana V; Departamento de Biologia Celular y Genetica, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico., Serna-Hernandez JC; Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo Leon, Mexico., Hernandez-Martinez SJ; Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo Leon, Mexico., Castelan-Maldonado EE; Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo Leon, Mexico., Montalvo-Bañuelos MS; Departamento de Foniatria, Hospital General de Zona No. 6, Instituto Mexicano del Seguro Social, San Nicolas de los Garza, Nuevo Leon, Mexico., Alonso-Tellez CA; Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo Leon, Mexico., Sanchez-Fresno EC; Division de Investigacion, Departamento de Otorrinolaringologia y Departamento de Anatomia Patologica, Unidad Medica de Alta Especialidad, Hospital de Especialidades No. 25, Instituto Mexicano del Seguro Social, Monterrey, Nuevo Leon, Mexico., Tamez-Guerra RS; Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico., Rodriguez-Padilla C; Laboratorio de Inmunologia y Virologia, Facultad de Ciencias Biologicas, Universidad Autonoma de Nuevo Leon, San Nicolas de los Garza, Nuevo Leon, Mexico.
Jazyk: angličtina
Zdroj: Infectious agents and cancer [Infect Agent Cancer] 2023 Jul 11; Vol. 18 (1), pp. 43. Date of Electronic Publication: 2023 Jul 11.
DOI: 10.1186/s13027-023-00514-x
Abstrakt: Background: Laryngeal squamous cell carcinoma (LSCC) represents one of the principal tumors of the head and neck. Human papillomavirus (HPV) and Epstein-Barr virus (EBV) are considered risk factors for the development and the clinical prognosis of LSCC. High levels of p16 INK4a are suggested as a surrogate marker of HPV or EBV infection in some head and neck tumors but in LSCC is still controversial. Furthermore, pRb expression may be considered an additional biomarker but it has not been clearly defined. This work aimed to compare the expression of pRb and p16 INK4a as possible biomarkers in tumor tissues with and without infection by EBV or different genotypes of HPV from patients with LSCC.
Methods: Tumor samples from 103 patients with LSCC were previously investigated for the presence and genotypes of HPV using the INNO-LiPA line probe assay and for the infection of EBV by qPCR. p16 INK4a and pRb expression was assessed by immunohistochemistry.
Results: Of the 103 tumor samples, expression of p16 INK4a was positive in 55 (53.4%) and of this, 32 (56.1%) were positive for HPV whereas 11 (39.3%) were EBV positive but both without a significantly difference (p > 0.05). pRb expression was positive in 78 (75.7%) and a higher frequency of this expression was observed in HPV negative samples (87.0%) (p = 0.021) and in high-risk HPV negative samples (85.2%) (p = 0.010). No difference was observed when comparing pRb expression and EBV infection status (p > 0.05).
Conclusion: Our results support the suggestion that p16 INK4a is not a reliable surrogate marker for identifying HPV or EBV infection in LSCC. On the other hand, most of our samples had pRb expression, which was more frequent in tumors without HPV, suggesting that pRb could indicate HPV negativity. However, more studies with a larger number of cases are required, including controls without LSCC and evaluating other molecular markers to determine the real role of p16 INK4a and pRb in LSCC.
(© 2023. The Author(s).)
Databáze: MEDLINE
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