Bioconversion of vitamin D 3 into calcitriol by Actinomyces hyovaginalis isolate CCASU- A11-2.

Autor: Abbas AM; Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St. Abbassia, Cairo, 11566, Egypt.; Department of Microbiology & Immunology, Faculty of Pharmacy, King Salman International University, South Sinai, Egypt., Elkhatib WF; Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St. Abbassia, Cairo, 11566, Egypt.; Department of Microbiology & Immunology, Faculty of Pharmacy, Galala University, New Galala city, Suez, Egypt., Aboulwafa MM; Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St. Abbassia, Cairo, 11566, Egypt.; Department of Microbiology & Immunology, Faculty of Pharmacy, King Salman International University, South Sinai, Egypt., Hassouna NA; Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St. Abbassia, Cairo, 11566, Egypt., Aboshanab KM; Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization St. Abbassia, Cairo, 11566, Egypt. aboshanab2012@pharma.asu.edu.eg.
Jazyk: angličtina
Zdroj: AMB Express [AMB Express] 2023 Jul 12; Vol. 13 (1), pp. 73. Date of Electronic Publication: 2023 Jul 12.
DOI: 10.1186/s13568-023-01574-3
Abstrakt: Vitamin D 3 is a fat-soluble prohormone that is activated inside the liver to produce 25-hydroxyvitamin D 3 (calcidiol), and in the kidney to produce the fully active 1α, 25-dihydroxy vitamin D 3 (calcitriol). A previous work piloted in our laboratory, resulted in a successful recovery of a local soil-promising Actinomyces hyovaginalis isolate CCASU-A11-2 capable of converting vitamin D 3 into calcitriol. Despite the rising amount of research on vitamin D 3 bioconversion into calcitriol, further deliberate studies on this topic can significantly contribute to the improvement of such a bioconversion process. Therefore, this work aimed to improve the bioconversion process, using the study isolate, in a 14 L laboratory fermenter (4 L fermentation medium composed of fructose (15 g/L), defatted soybean (15 g/L), NaCl (5 g/L), CaCO 3 2 g/L); K 2 HPO 4 , (1 g/L) NaF (0.5 g/L) and initial of pH 7.8) where different experiments were undertaken to investigate the effect of different culture conditions on the bioconversion process. Using the 14 L laboratory fermenter, the calcitriol production was increased by about 2.5-fold (32.8 µg/100 mL) to that obtained in the shake flask (12.4 µg/100 mL). The optimal bioconversion conditions were inoculum size of 2% v/v, agitation rate of 200 rpm, aeration rate of 1 vvm, initial pH of 7.8 (uncontrolled); addition of vitamin D 3 (substrate) 48 h after the start of the main culture. In conclusion, the bioconversion of vitamin D 3 into calcitriol in a laboratory fermenter showed a 2.5-fold increase as compared to the shake flask level where, the important factors influencing the bioconversion process were the aeration rate, inoculum size, the timing of substrate addition, and the fixed pH of the fermentation medium. So, those factors should be critically considered for the scaling-up of the biotransformation process.
(© 2023. The Author(s).)
Databáze: MEDLINE
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