Staphylococcus aureus Biofilm Destabilization by Tween-80 and Lung Surfactants to Overcome Biofilm-Imposed Drug Resistance.

Autor: Maurya S; Centre of Biotechnology, Faculty of Science, University of Allahabad, Prayagraj, Uttar Pradesh, 211002, India., Gaur M; Centre of Biotechnology, Faculty of Science, University of Allahabad, Prayagraj, Uttar Pradesh, 211002, India., Yadav AB; Centre of Biotechnology, Faculty of Science, University of Allahabad, Prayagraj, Uttar Pradesh, 211002, India. awadhyadav@allduniv.ac.in.
Jazyk: angličtina
Zdroj: Applied biochemistry and biotechnology [Appl Biochem Biotechnol] 2024 Mar; Vol. 196 (3), pp. 1558-1569. Date of Electronic Publication: 2023 Jul 11.
DOI: 10.1007/s12010-023-04650-3
Abstrakt: This study is aimed to evaluating the potential of tween-80 and artificial lung surfactant (ALS) to destabilize S. aureus biofilm. The biofilm destabilization was studied by crystal violet staining, bright field microscopy, and scanning electron microscopy (SEM). During the study, S. aureus biofilm was exposed with tween-80 along various concentrations (1%, 0.1%, and 0.05%) or LS (lung surfactant) at (2.5%, 5%, and 15%) for 2 hrs. It was observed that 0.1% of tween-80 destabilized 63.83 ± 4.35% and 15% ALS 77 ± 1.7% biofilm in comparison to without treatment. The combination of tween-80 and ALS was used and showed a synergistic effect to destabilize 83.4 ± 1.46% biofilm. These results showed the potential of tween-80 and ALS as biofilm disruptors, which further needs to explore in an in-vivo animal model to access the actual potential of biofilm disruption in natural conditions. This study could play a pivotal role to overcome the problem of antibiotic resistance imposed due to biofilm formation to combat antibiotic resistance imposed by bacteria.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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