Peek-A-Boo Test: A Simple Test for Assessing the Effect of Anxiolytics on Fish Behavior.

Autor: Takai Y; Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan., Izumi M; Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan., Motoyama Y; Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan., Shimasaki Y; Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan., Oshima Y; Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.; Institute of Nature and Environmental Technology, Kanazawa University, Ishikawa, Japan., Kang IJ; School of Interdisciplinary Science and Innovation, Kyushu University, Fukuoka, Japan.
Jazyk: angličtina
Zdroj: Environmental toxicology and chemistry [Environ Toxicol Chem] 2023 Nov; Vol. 42 (11), pp. 2358-2363. Date of Electronic Publication: 2023 Aug 17.
DOI: 10.1002/etc.5713
Abstrakt: The potential of pharmaceuticals and personal care products to alter the behavior of aquatic organisms is a growing concern. To assess the actual effect of these substances on aquatic organisms, a simple but effective behavioral test is required. We devised a simple behavioral (Peek-A-Boo) test to assess the effect of anxiolytics on the behavior of a model fish (medaka, Oryzias latipes). In the Peek-A-Boo test, we investigated the response of medaka to an image of a predator fish (donko fish, Odontobutis obscura). The test revealed that the time taken for test medaka exposed to diazepam (0.8, 4, 20, or 100 µg/L) to approach the image was shorter by a factor of 0.22 to 0.65, and the time spent in the area close to the image was longer by a factor of 1.8 to 2.7 than in the solvent control group for all diazepam exposure groups (p < 0.05). Hence, we confirmed that the test could detect changes in medaka behavior caused by diazepam with high sensitivity. The Peek-A-Boo test we devised is a simple behavioral test with high sensitivity for fish behavioral alteration. Environ Toxicol Chem 2023;42:2358-2363. © 2023 SETAC.
(© 2023 SETAC.)
Databáze: MEDLINE
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