Detection of TALEN-mediated genome cleavage during the early embryonic stage of the starfish Patiria pectinifera.

Autor: Yamakawa S; Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan., Sasakura Y; Shimoda Marine Research Center, University of Tsukuba, Shimoda, Shizuoka, Japan., Morino Y; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan., Wada H; Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Jazyk: angličtina
Zdroj: Developmental dynamics : an official publication of the American Association of Anatomists [Dev Dyn] 2023 Dec; Vol. 252 (12), pp. 1471-1481. Date of Electronic Publication: 2023 Jul 11.
DOI: 10.1002/dvdy.641
Abstrakt: Background: Echinoderms have long been utilized as experimental materials to study the genetic control of developmental processes and their evolution. Among echinoderms, the molecular study of starfish embryos has received considerable attention across research topics such as gene regulatory network evolution and larval regeneration. Recently, experimental techniques to manipulate gene functions have been gradually established in starfish as the feasibility of genome editing methods was reported. However, it is still unclear when these techniques cause genome cleavage during the development of starfish, which is critical to understand the timeframe and applicability of the experiment during early development of starfish.
Results: We herein reported that gene functions can be analyzed by the genome editing method TALEN in early embryos, such as the blastula of the starfish Patiria pectinifera. We injected the mRNA of TALEN targeting rar, which was previously constructed, into eggs of P. pectinifera and examined the efficiency of genome cleavage through developmental stages from 6 to 48 hours post fertilization.
Conclusion: The results will be key knowledge not only when designing TALEN-based experiments but also when assessing the results.
(© 2023 American Association for Anatomy.)
Databáze: MEDLINE
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