Enhancement of dielectrophoresis-based particle collection from high conducting fluids due to partial electrode insulation.

Autor: Luna R; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA.; Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, Oregon, USA., Heineck D; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA., Hinestrosa JP; Biological Dynamics, San Diego, California, USA., Dobrovolskaia I; Biological Dynamics, San Diego, California, USA., Hamilton S; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA.; Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, Oregon, USA., Malakian A; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA., Gustafson KT; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA.; Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, Oregon, USA., Huynh KT; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA.; Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, Oregon, USA., Kim S; School of Chemical Engineering, Clean Energy Research Center, Jeonbuk National University, Jeonju-si, Jeollabuk-do, South Korea., Ware J; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA.; Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, Oregon, USA., Stimson E; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA.; Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, Oregon, USA., Ross C; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA., Schutt CE; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA.; Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, Oregon, USA., Ibsen SD; Cancer Early Detection Advanced Research Center, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USA.; Department of Biomedical Engineering, School of Medicine, Oregon Health and Science University, Portland, Oregon, USA.
Jazyk: angličtina
Zdroj: Electrophoresis [Electrophoresis] 2023 Aug; Vol. 44 (15-16), pp. 1234-1246. Date of Electronic Publication: 2023 Jul 10.
DOI: 10.1002/elps.202200295
Abstrakt: Dielectrophoresis (DEP) is a successful method to recover nanoparticles from different types of fluid. The DEP force acting on these particles is created by an electrode microarray that produces a nonuniform electric field. To apply DEP to a highly conducting biological fluid, a protective hydrogel coating over the metal electrodes is required to create a barrier between the electrode and the fluid. This protects the electrodes, reduces the electrolysis of water, and allows the electric field to penetrate into the fluid sample. We observed that the protective hydrogel layer can separate from the electrode and form a closed domed structure and that collection of 100 nm polystyrene beads increased when this occurred. To better understand this collection increase, we used COMSOL Multiphysics software to model the electric field in the presence of the dome filled with different materials ranging from low-conducting gas to high conducting phosphate-buffered saline fluids. The results suggest that as the electrical conductivity of the material inside the dome is reduced, the whole dome acts as an insulator which increases electric field intensity at the electrode edge. This increased intensity widens the high-intensity electric field factor zone resulting in increased collection. This informs how dome formation results in increased particle collection and provides insight into how the electric field can be intensified to the increase collection of particles. These results have important applications for increasing the recovery of biologically-derived nanoparticles from undiluted physiological fluids that have high conductance, including the collection of cancer-derived extracellular vesicles from plasma for liquid biopsy applications.
(© 2023 The Authors. Electrophoresis published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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