Lipid extraction and analysis of microalgae strain pectinodesmus PHM3 for biodiesel production.

Autor: Kiyani DA; Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology (NUST), Islamabad, 44000, Pakistan., Maryam S; Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology (NUST), Islamabad, 44000, Pakistan., Amina SJ; Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology (NUST), Islamabad, 44000, Pakistan., Ahmad A; Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology (NUST), Islamabad, 44000, Pakistan., Chattha MWA; Department of Plant Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology (NUST), Islamabad, 44000, Pakistan., Janjua HA; Department of Industrial Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology (NUST), Islamabad, 44000, Pakistan. janjua.hussnain@gmail.com.
Jazyk: angličtina
Zdroj: BMC biotechnology [BMC Biotechnol] 2023 Jul 10; Vol. 23 (1), pp. 20. Date of Electronic Publication: 2023 Jul 10.
DOI: 10.1186/s12896-023-00784-8
Abstrakt: The current study is focused on the lipid extract of microalgae; Pectinodesmus strain PHM3 and its general analysis in terms of chemical contents. Combinations of both chemical and mechanistic approaches were applied to obtain the maximum yield of lipids which was recorded to be 23% per gram through continuous agitation using Folch solution. The extraction methods used in this study included: Bligh and Dyers method, Continuous agitation method, Extraction using Soxhlet and Acid base extraction method. Lipid quantification of ethanol and Folch solution lipid extract was performed through gravimetric methods and qualification was done through Fourier Transmission Infrared Spectroscopy (FTIR) and Gas Chromatographymass spectrometry (GC-MS). Phytochemical analysis identified other compounds in ethanol extract and the results confirmed the presence of steroids, coumarins, tannins, phenols and carbohydrates. Transesterification of lipids showed 7% per gram dry weight yield of Pectinodesmus PHM3. GC-MS studies of extracted biodiesel suggested that 72% of biofuels was in the form of dipropyl ether, ethyl butyl ethers, methyl butyl ether and propyl butyl ether. Lipid processing of acid-base extract showed that oily nature of lipid shifted to a more precipitated form which is a common observation when mixture of lipids is converted to phosphatides.
(© 2023. The Author(s).)
Databáze: MEDLINE
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