Whole-body cellular mapping in mouse using standard IgG antibodies.

Autor: Mai H; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany.; Institute for Stroke and Dementia Research, Medical Centre of the University of Munich, Ludwig-Maximilians University of Munich, Munich, Germany.; Munich Medical Research School, Munich, Germany.; Deep Piction GmbH, Munich, Germany., Luo J; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany.; Institute for Stroke and Dementia Research, Medical Centre of the University of Munich, Ludwig-Maximilians University of Munich, Munich, Germany.; Deep Piction GmbH, Munich, Germany., Hoeher L; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany., Al-Maskari R; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany.; TUM School of Computation, Information and Technology, Technical University of Munich, Munich, Germany., Horvath I; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany.; TUM School of Computation, Information and Technology, Technical University of Munich, Munich, Germany., Chen Y; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany.; Institute for Stroke and Dementia Research, Medical Centre of the University of Munich, Ludwig-Maximilians University of Munich, Munich, Germany.; Faculty of Medicine, Ludwig-Maximilians University of Munich, Munich, Germany., Kofler F; Helmholtz Al, Helmholtz Center Munich, Neuherberg, Germany.; Department of Informatics, Technical University of Munich, Munich, Germany.; TranslaTUM - Central Institute for Translational Cancer Research, Technical University of Munich, Munich, Germany.; Department of Diagnostic and Interventional Neuroradiology, School of Medicine, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany., Piraud M; Helmholtz Al, Helmholtz Center Munich, Neuherberg, Germany., Paetzold JC; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany.; Department of Computing, Imperial College London, London, UK., Modamio J; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany., Todorov M; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany.; Institute for Stroke and Dementia Research, Medical Centre of the University of Munich, Ludwig-Maximilians University of Munich, Munich, Germany.; Munich Cluster for Systems Neurology (SyNergy), Munich, Germany., Elsner M; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany., Hellal F; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany.; Institute for Stroke and Dementia Research, Medical Centre of the University of Munich, Ludwig-Maximilians University of Munich, Munich, Germany.; Munich Cluster for Systems Neurology (SyNergy), Munich, Germany., Ertürk A; Institute for Tissue Engineering and Regenerative Medicine, Helmholtz Center Munich, Neuherberg, Germany. ali.erturk@helmholtz-munich.de.; Institute for Stroke and Dementia Research, Medical Centre of the University of Munich, Ludwig-Maximilians University of Munich, Munich, Germany. ali.erturk@helmholtz-munich.de.; Deep Piction GmbH, Munich, Germany. ali.erturk@helmholtz-munich.de.; Munich Cluster for Systems Neurology (SyNergy), Munich, Germany. ali.erturk@helmholtz-munich.de.; Graduate School of Neuroscience (GSN), Munich, Germany. ali.erturk@helmholtz-munich.de.
Jazyk: angličtina
Zdroj: Nature biotechnology [Nat Biotechnol] 2024 Apr; Vol. 42 (4), pp. 617-627. Date of Electronic Publication: 2023 Jul 10.
DOI: 10.1038/s41587-023-01846-0
Abstrakt: Whole-body imaging techniques play a vital role in exploring the interplay of physiological systems in maintaining health and driving disease. We introduce wildDISCO, a new approach for whole-body immunolabeling, optical clearing and imaging in mice, circumventing the need for transgenic reporter animals or nanobody labeling and so overcoming existing technical limitations. We identified heptakis(2,6-di-O-methyl)-β-cyclodextrin as a potent enhancer of cholesterol extraction and membrane permeabilization, enabling deep, homogeneous penetration of standard antibodies without aggregation. WildDISCO facilitates imaging of peripheral nervous systems, lymphatic vessels and immune cells in whole mice at cellular resolution by labeling diverse endogenous proteins. Additionally, we examined rare proliferating cells and the effects of biological perturbations, as demonstrated in germ-free mice. We applied wildDISCO to map tertiary lymphoid structures in the context of breast cancer, considering both primary tumor and metastases throughout the mouse body. An atlas of high-resolution images showcasing mouse nervous, lymphatic and vascular systems is accessible at http://discotechnologies.org/wildDISCO/atlas/index.php .
(© 2023. The Author(s).)
Databáze: MEDLINE
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