A positively tuned voltage indicator for extended electrical recordings in the brain.

Autor: Evans SW; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Shi DQ; Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China., Chavarha M; Department of Bioengineering, Stanford University, Stanford, CA, USA., Plitt MH; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Taxidis J; Department of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA, USA.; Program in Neurosciences & Mental Health, Hospital for Sick Children, Toronto, Ontario, Canada.; Department of Physiology, University of Toronto, Toronto, Ontario, Canada.; Program in Neurosciences & Mental Health, Hospital for Sick Children, Toronto, Ontario, Canada.; Department of Physiology, University of Toronto, Toronto, Ontario, Canada., Madruga B; Department of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA, USA., Fan JL; UC Berkeley/UCSF Joint Program in Bioengineering, University of California Berkeley, Berkeley, CA, USA., Hwang FJ; Department of Neurosurgery, Stanford University Medical Center, Stanford, CA, USA., van Keulen SC; Department of Computer Science, Stanford University, Stanford, CA, USA., Suomivuori CM; Department of Computer Science, Stanford University, Stanford, CA, USA., Pang MM; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Su S; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Lee S; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Hao YA; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA.; Department of Bioengineering, Stanford University, Stanford, CA, USA., Zhang G; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Jiang D; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Pradhan L; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Roth RH; Department of Neurosurgery, Stanford University Medical Center, Stanford, CA, USA., Liu Y; Department of Neurosurgery, Stanford University Medical Center, Stanford, CA, USA.; Department of Ophthalmology, Stanford University Medical Center, Stanford, CA, USA., Dorian CC; Department of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA, USA., Reese AL; Institute for Genomic Medicine, Columbia University, New York, NY, USA., Negrean A; Department of Neuroscience, Columbia University, New York, NY, USA., Losonczy A; Department of Neuroscience, Columbia University, New York, NY, USA.; Mortimer B. Zuckerman Mind Brain Behavior Institute, New York, NY, USA.; Kavli Institute for Brain Science, New York, NY, USA., Makinson CD; Institute for Genomic Medicine, Columbia University, New York, NY, USA.; Department of Neurology, Columbia University, New York, NY, USA., Wang S; Department of Ophthalmology, Stanford University Medical Center, Stanford, CA, USA., Clandinin TR; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Dror RO; Department of Computer Science, Stanford University, Stanford, CA, USA.; Department of Structural Biology, Stanford University School of Medicine, Stanford, CA, USA.; Institute for Computational and Mathematical Engineering, Stanford University, Stanford, CA, USA.; Department of Molecular and Cellular Physiology, Stanford University, Stanford, USA., Ding JB; Department of Neurosurgery, Stanford University Medical Center, Stanford, CA, USA.; Department of Neurology and Neurological Sciences, Stanford University Medical Center, Stanford, CA, USA., Ji N; Program in Neurosciences & Mental Health, Hospital for Sick Children, Toronto, Ontario, Canada.; Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, USA.; Department of Physics, University of California Berkeley, Berkeley, CA, USA.; Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA, USA.; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA., Golshani P; Department of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA, USA.; Semel Institute for Neuroscience and Human Behavior, UCLA David Geffen School of Medicine, Los Angeles, CA, USA., Giocomo LM; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA., Bi GQ; Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.; Interdisciplinary Center for Brain Information, Brain Cognition and Brain Disease Institute, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China., Lin MZ; Department of Neurobiology, Stanford University Medical Center, Stanford, CA, USA. mzlin@stanford.edu.; Department of Bioengineering, Stanford University, Stanford, CA, USA. mzlin@stanford.edu.; Department of Chemical and Systems Biology, Stanford University, Stanford, USA. mzlin@stanford.edu.
Jazyk: angličtina
Zdroj: Nature methods [Nat Methods] 2023 Jul; Vol. 20 (7), pp. 1104-1113. Date of Electronic Publication: 2023 Jul 06.
DOI: 10.1038/s41592-023-01913-z
Abstrakt: Genetically encoded voltage indicators (GEVIs) enable optical recording of electrical signals in the brain, providing subthreshold sensitivity and temporal resolution not possible with calcium indicators. However, one- and two-photon voltage imaging over prolonged periods with the same GEVI has not yet been demonstrated. Here, we report engineering of ASAP family GEVIs to enhance photostability by inversion of the fluorescence-voltage relationship. Two of the resulting GEVIs, ASAP4b and ASAP4e, respond to 100-mV depolarizations with ≥180% fluorescence increases, compared with the 50% fluorescence decrease of the parental ASAP3. With standard microscopy equipment, ASAP4e enables single-trial detection of spikes in mice over the course of minutes. Unlike GEVIs previously used for one-photon voltage recordings, ASAP4b and ASAP4e also perform well under two-photon illumination. By imaging voltage and calcium simultaneously, we show that ASAP4b and ASAP4e can identify place cells and detect voltage spikes with better temporal resolution than commonly used calcium indicators. Thus, ASAP4b and ASAP4e extend the capabilities of voltage imaging to standard one- and two-photon microscopes while improving the duration of voltage recordings.
(© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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