A draft reference genome assembly of the Pipevine Swallowtail butterfly, Battus philenor hirsuta.

Autor:	Chaturvedi S; Department of Integrative Biology, University of California, 142 Weill Hall #3200, Berkeley, CA 94720, United States.; Department of Ecology and Evolutionary Biology, Tulane University, New Orleans, LA 70118, United States., Escalona M; Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, United States., Marimuthu MPA; DNA Technologies and Expression Analysis Core Laboratory, Genome Center, University of California, Davis, CA 95616, United States., Nguyen O; DNA Technologies and Expression Analysis Core Laboratory, Genome Center, University of California, Davis, CA 95616, United States., Chumchim N; DNA Technologies and Expression Analysis Core Laboratory, Genome Center, University of California, Davis, CA 95616, United States., Fairbairn CW; Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, United States., Seligmann W; Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, Santa Cruz, CA 95064, United States., Miller C; Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA 90095-7239, United States., Bradley Shaffer H; Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA 90095-7239, United States.; La Kretz Center for California Conservation Science, Institute of the Environment and Sustainability, University of California, Los Angeles, CA 90095-7239, United States., Whiteman NK; Department of Integrative Biology, University of California, 142 Weill Hall #3200, Berkeley, CA 94720, United States.; Department of Molecular and Cell Biology, University of California, 142 Weill Hall #3200, Berkeley, CA 94720, United States.
Jazyk:	angličtina
Zdroj:	The Journal of heredity [J Hered] 2023 Nov 15; Vol. 114 (6), pp. 698-706.
DOI:	10.1093/jhered/esad043
Abstrakt:	The California Pipevine Swallowtail Butterfly, Battus philenor hirsuta, and its host plant, the California Pipevine or Dutchman's Pipe, Aristolochia californica Torr., are an important California endemic species pair. While this species pair is an ideal system to study co-evolution, genomic resources for both are lacking. Here, we report a new, chromosome-level assembly of B. philenor hirsuta as part of the California Conservation Genomics Project (CCGP). Following the sequencing and assembly strategy of the CCGP, we used Pacific Biosciences HiFi long reads and Hi-C chromatin proximity sequencing technology to produce a de novo assembled genome. Our genome assembly, the first for any species in the genus, contains 109 scaffolds spanning 443 mega base (Mb) pairs, with a contig N50 of 14.6 Mb, a scaffold N50 of 15.2 Mb, and BUSCO complete score of 98.9%. In combination with the forthcoming A. californica reference genome, the B. philenor hirsuta genome will be a powerful tool for documenting landscape genomic diversity and plant-insect co-evolution in a rapidly changing California landscape. (© The American Genetic Association. 2023.)
Databáze:	MEDLINE
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