Inflammatory and tolerogenic myeloid cells determine outcome following human allergen challenge.

Autor:	Voskamp AL; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Tak T; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Gerdes ML; Department of Ear, Nose and Throat, Erasmus University Medical Center, Rotterdam, Netherlands., Menafra R; Leiden Genome Technology Center, Leiden University Medical Center , Leiden, Netherlands., Duijster E; Department of Internal Medicine, Section Allergology and Clinical Immunology, Erasmus University Medical Center , Rotterdam, Netherlands., Jochems SP; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., Kielbasa SM; Department of Biomedical Data Sciences, Leiden University Medical Center, Leiden, Netherlands., Kormelink TG; Department of Exp Immunology, Amsterdam University Medical Centers, Amsterdam, Netherlands., Stam KA; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., van Hengel ORJ; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., de Jong NW; Department of Internal Medicine, Section Allergology and Clinical Immunology, Erasmus University Medical Center , Rotterdam, Netherlands., Hendriks RW; Department of Pulmonary Medicine, Erasmus University Medical Center, Rotterdam, Netherlands., Kloet SL; Leiden Genome Technology Center, Leiden University Medical Center , Leiden, Netherlands., Yazdanbakhsh M; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands., de Jong EC; Department of Exp Immunology, Amsterdam University Medical Centers, Amsterdam, Netherlands., Gerth van Wijk R; Department of Internal Medicine, Section Allergology and Clinical Immunology, Erasmus University Medical Center , Rotterdam, Netherlands., Smits HH; Department of Parasitology, Leiden University Medical Center, Leiden, Netherlands.
Jazyk:	angličtina
Zdroj:	The Journal of experimental medicine [J Exp Med] 2023 Sep 04; Vol. 220 (9). Date of Electronic Publication: 2023 Jul 10.
DOI:	10.1084/jem.20221111
Abstrakt:	Innate mononuclear phagocytic system (MPS) cells preserve mucosal immune homeostasis. We investigated their role at nasal mucosa following allergen challenge with house dust mite. We combined single-cell proteome and transcriptome profiling on nasal immune cells from nasal biopsies cells from 30 allergic rhinitis and 27 non-allergic subjects before and after repeated nasal allergen challenge. Biopsies of patients showed infiltrating inflammatory HLA-DRhi/CD14+ and CD16+ monocytes and proallergic transcriptional changes in resident CD1C+/CD1A+ conventional dendritic cells (cDC)2 following challenge. In contrast, non-allergic individuals displayed distinct innate MPS responses to allergen challenge: predominant infiltration of myeloid-derived suppressor cells (MDSC: HLA-DRlow/CD14+ monocytes) and cDC2 expressing inhibitory/tolerogenic transcripts. These divergent patterns were confirmed in ex vivo stimulated MPS nasal biopsy cells. Thus, we identified not only MPS cell clusters involved in airway allergic inflammation but also highlight novel roles for non-inflammatory innate MPS responses by MDSC to allergens in non-allergic individuals. Future therapies should address MDSC activity as treatment for inflammatory airway diseases. (© 2023 Voskamp et al.)
Databáze:	MEDLINE
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