Ultramicromethod for measuring the activity of terminal deoxynucleotidyl transferase.

Autor: Léotard B, Marchand-Arvier M, Vigneron C
Jazyk: angličtina
Zdroj: Clinical chemistry [Clin Chem] 1986 Sep; Vol. 32 (9), pp. 1779-81.
Abstrakt: We describe an ultramicromethod for measurement of terminal deoxynucleotidyl transferase activity. The materials used are as proposed by Hösli (Clin Chem 1977;23:1476-84) for antenatal diagnosis. The technique is based on the methods of Beutler and Kuhl (Am J Clin Pathol 1978;70:733-7) and Yasmineh et al. (Clin Chem 1980; 26:891-5). The procedure makes it possible to reduce the blood sample to 2 mL at the most and to reduce the cost of the measurement very considerably. The technique gives results similar to those given by the conventional techniques.
Databáze: MEDLINE